Literature summary extracted from
Suyotha, W.; Fujiki, H.; Cherdvorapong, V.; Takagi, K.; Yano, S.; Wakayama, M.
A novel thermostable alpha-1,3-glucanase from Streptomyces thermodiastaticus HF 3-3 (2017), J. Gen. Appl. Microbiol., 63, 296-304 .
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.2.1.59 |
dithiothreitol |
124% activity at 1 mM |
Streptomyces thermodiastaticus |
|
General Stability
EC Number |
General Stability |
Organism |
---|
3.2.1.59 |
the enzyme is stable in the presence of chemicals like SDS, benzethonium chloride, and sodium fluoride at a concentration of 1% (w/v). The enzyme also exhibits salt tolerance at a concentration up to 20% (w/v) |
Streptomyces thermodiastaticus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.59 |
Ag+ |
less than 20% residual activity at 1 mM |
Streptomyces thermodiastaticus |
|
3.2.1.59 |
Cu2+ |
80% residual activity at 1 mM |
Streptomyces thermodiastaticus |
|
3.2.1.59 |
EDTA |
about 80% residual activity at 1 mM |
Streptomyces thermodiastaticus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.2.1.59 |
Ca2+ |
125% activity at 1 mM |
Streptomyces thermodiastaticus |
|
3.2.1.59 |
Co2+ |
160% activity at 1 mM |
Streptomyces thermodiastaticus |
|
3.2.1.59 |
Li+ |
115% activity at 1 mM |
Streptomyces thermodiastaticus |
|
3.2.1.59 |
Mn2+ |
124% activity at 1 mM |
Streptomyces thermodiastaticus |
|
3.2.1.59 |
additional information |
Na+, K+, NH4+, Mg2+, Fe2+, Zn2+, and Ni2+ do not significantly affect the enzyme activity |
Streptomyces thermodiastaticus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.59 |
45700 |
- |
gel filtration |
Streptomyces thermodiastaticus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.59 |
Streptomyces thermodiastaticus |
- |
- |
- |
3.2.1.59 |
Streptomyces thermodiastaticus HF 3-3 |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.59 |
ammonium sulfate precipitation, DEAE-cellulofine column chromatography and butyl-Toyopearl 650M column chromatography |
Streptomyces thermodiastaticus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.59 |
alpha-1,3-glucan + H2O |
the enzyme exhibits high specificity against alpha-1,3-glucan |
Streptomyces thermodiastaticus |
? |
- |
? |
|
3.2.1.59 |
alpha-1,3-glucan + H2O |
the enzyme exhibits high specificity against alpha-1,3-glucan |
Streptomyces thermodiastaticus HF 3-3 |
? |
- |
? |
|
3.2.1.59 |
additional information |
no activity with dextran, cellulose, amylose, amylopectin, and laminarin |
Streptomyces thermodiastaticus |
? |
- |
? |
|
3.2.1.59 |
additional information |
no activity with dextran, cellulose, amylose, amylopectin, and laminarin |
Streptomyces thermodiastaticus HF 3-3 |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.59 |
? |
x * 65000, SDS-PAGE |
Streptomyces thermodiastaticus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.59 |
alpha-1,3-glucanase HF65 |
- |
Streptomyces thermodiastaticus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.59 |
65 |
- |
- |
Streptomyces thermodiastaticus |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.2.1.59 |
50 |
70 |
more than 60% activity between 50 and 70°C |
Streptomyces thermodiastaticus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.59 |
65 |
70 |
the purified enzyme retains more than 50% of maximum activity after incubation at 65°C more than 2 h and exhibits about 95% of full activity after incubation at 65°C for 10 min. After treatment at 70°C for 120 min, the enzyme shows 20% residual activity |
Streptomyces thermodiastaticus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.59 |
5 |
5.5 |
- |
Streptomyces thermodiastaticus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.2.1.59 |
4 |
6.5 |
more than 60% activity between pH 4.0 and 6.5 |
Streptomyces thermodiastaticus |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
3.2.1.59 |
4.5 |
6.5 |
the purified enzyme retains more than 80% of maximum activity after 2 h at pH 4.5-6.5 |
Streptomyces thermodiastaticus |