EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | gene phyA, DNA and amino acid sequence determination and analysis, sequence comparisons | Penicillium oxalicum |
3.1.3.26 | gene phyA, DNA and amino acid sequence determination and analysis, sequence comparisons | Penicillium oxalicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Al3+ | - |
Penicillium oxalicum | |
3.1.3.8 | Ba2+ | - |
Penicillium oxalicum | |
3.1.3.8 | Ca2+ | - |
Penicillium oxalicum | |
3.1.3.8 | Cd2+ | - |
Penicillium oxalicum | |
3.1.3.8 | Co2+ | - |
Penicillium oxalicum | |
3.1.3.8 | Cu2+ | - |
Penicillium oxalicum | |
3.1.3.8 | EDTA | - |
Penicillium oxalicum | |
3.1.3.8 | Li+ | - |
Penicillium oxalicum | |
3.1.3.8 | Mg2+ | - |
Penicillium oxalicum | |
3.1.3.8 | Mn2+ | - |
Penicillium oxalicum | |
3.1.3.8 | additional information | poor inhibition by Co2+ and Al3+ at 5 mM | Penicillium oxalicum | |
3.1.3.8 | Zn2+ | - |
Penicillium oxalicum | |
3.1.3.26 | Al3+ | - |
Penicillium oxalicum | |
3.1.3.26 | Ba2+ | - |
Penicillium oxalicum | |
3.1.3.26 | Ca2+ | - |
Penicillium oxalicum | |
3.1.3.26 | Cd2+ | - |
Penicillium oxalicum | |
3.1.3.26 | Co2+ | - |
Penicillium oxalicum | |
3.1.3.26 | Cu2+ | - |
Penicillium oxalicum | |
3.1.3.26 | EDTA | - |
Penicillium oxalicum | |
3.1.3.26 | Li+ | - |
Penicillium oxalicum | |
3.1.3.26 | Mg2+ | - |
Penicillium oxalicum | |
3.1.3.26 | Mn2+ | - |
Penicillium oxalicum | |
3.1.3.26 | additional information | poor inhibition by Co2+ and Al3+ at 5 mM | Penicillium oxalicum | |
3.1.3.26 | Zn2+ | - |
Penicillium oxalicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | additional information | - |
additional information | Michaelis-Menten kinetics and Lineweaver-Burk plots | Penicillium oxalicum | |
3.1.3.8 | 0.48 | 0.545 | myo-inositol hexakisphosphate | pH 5.5, 55°C, sodium phytate | Penicillium oxalicum | |
3.1.3.26 | additional information | - |
additional information | Michaelis-Menten kinetics and Lineweaver-Burk plots | Penicillium oxalicum | |
3.1.3.26 | 0.48 | 0.545 | myo-inositol hexakisphosphate | pH 5.5, 55°C, sodium phytate | Penicillium oxalicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.8 | extracellular | - |
Penicillium oxalicum | - |
- |
3.1.3.26 | extracellular | - |
Penicillium oxalicum | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Penicillium oxalicum | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Penicillium oxalicum KCTC6440 | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | Penicillium oxalicum | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | Penicillium oxalicum KCTC6440 | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Penicillium oxalicum | D9I0I9 | - |
- |
3.1.3.8 | Penicillium oxalicum KCTC6440 | D9I0I9 | - |
- |
3.1.3.26 | Penicillium oxalicum | D9I0I9 | - |
- |
3.1.3.26 | Penicillium oxalicum KCTC6440 | D9I0I9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | native extracellular enzyme from culture medium 486.41fold by ammonium sulfate fractionation, cation and anion exchange chromatography, and dialysis | Penicillium oxalicum |
3.1.3.26 | native extracellular enzyme from culture medium 486.41fold by ammonium sulfate fractionation, cation and anion exchange chromatography, and dialysis | Penicillium oxalicum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 447.5 | - |
purified native enzyme, pH 5.5, 55°C | Penicillium oxalicum |
3.1.3.26 | 447.5 | - |
purified native enzyme, pH 5.5, 55°C | Penicillium oxalicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP | Penicillium oxalicum | ? | - |
? | |
3.1.3.8 | additional information | enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP | Penicillium oxalicum KCTC6440 | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium oxalicum | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, substrate is sodium phytate | Penicillium oxalicum | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium oxalicum KCTC6440 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | additional information | enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP | Penicillium oxalicum | ? | - |
? | |
3.1.3.26 | additional information | enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP | Penicillium oxalicum KCTC6440 | ? | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium oxalicum | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, substrate is sodium phytate | Penicillium oxalicum | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium oxalicum KCTC6440 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, substrate is sodium phytate | Penicillium oxalicum KCTC6440 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | ? | x * 65000, SDS-PAGE | Penicillium oxalicum |
3.1.3.26 | ? | x * 65000, SDS-PAGE | Penicillium oxalicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | More | cf. EC 3.1.3.26 | Penicillium oxalicum |
3.1.3.8 | PhyA | - |
Penicillium oxalicum |
3.1.3.26 | More | cf. EC 3.1.3.8 | Penicillium oxalicum |
3.1.3.26 | PhyA | - |
Penicillium oxalicum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 55 | - |
- |
Penicillium oxalicum |
3.1.3.26 | 55 | - |
- |
Penicillium oxalicum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 25 | 70 | activity range, profile overview | Penicillium oxalicum |
3.1.3.26 | 25 | 70 | activity range, profile overview | Penicillium oxalicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5.5 | - |
- |
Penicillium oxalicum |
3.1.3.26 | 5.5 | - |
- |
Penicillium oxalicum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 3 | 7 | activity range, profile overview. Over 70% of maximal activity at pH 4.5-6.5 | Penicillium oxalicum |
3.1.3.26 | 3 | 7 | activity range, profile overview. Over 70% of maximal activity at pH 4.5-6.5 | Penicillium oxalicum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 4.5 | 6.5 | purified enzyme, stable at | Penicillium oxalicum |
3.1.3.26 | 4.5 | 6.5 | purified enzyme, stable at | Penicillium oxalicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.8 | additional information | homology modeling and computational analysis of Penicillium oxalicum strain KCTC6440 PhyA, comparison to the structure of phytase PhyA from Penicillium oxalicum strain PJ3 | Penicillium oxalicum |
3.1.3.26 | additional information | homology modeling and computational analysis of Penicillium oxalicum strain KCTC6440 PhyA, comparison to the structure of phytase PhyA from Penicillium oxalicum strain PJ3 | Penicillium oxalicum |