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Literature summary extracted from

  • Lockridge, O.; David, E.; Schopfer, L.M.; Masson, P.; Brazzolotto, X.; Nachon, F.
    Purification of recombinant human butyrylcholinesterase on Hupresin? (2018), J. Chromatogr. B, 1102-1103, 109-115 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8 recombinant expression of truncated human enzyme in stably transfected CHO-K1 cells, the recombinant enzyme is secreted Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 additional information generation of a truncated L530stop, 4 sugars off HuBChE mutant. The deleted glycosylation sites N17/455/481/486 unmask N485 for glycosylation, so that the rHuBChE contains 6 glycans. N485 is not glycated in plasma-derived HuBChE Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 huprine 19
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.8 extracellular
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.8 butyrylcholine + H2O Homo sapiens
-
 choline + butyrate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens P06276
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.8 glycoprotein the deleted glycosylation sites N17/455/481/486 unmask N485 for glycosylation, so that the recombinant HuBChE contains 6 glycans. N485 is not glycated in native plasma-derived HuBChE Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8 recombinant extracellular truncated human enzyme from CHO-K1 cell culture medium by Hupresin affinity chromatography, elution is best with 0.1M tetramethylammonium bromide in 20mM TrisCl, pH 7.5, followed by dialysis and ultrafiltration, to homogeneity. Loss of 24% during the dialysis and concentration steps Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 blood plasma
-
 Homo sapiens
-
3.1.1.8 serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 630
-
 purified recombinant truncated enzyme, pH 7.0, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 butyrylcholine + H2O
-
 Homo sapiens choline + butyrate
-
 ?
3.1.1.8 butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.8 tetramer native plasma-derived HuBChE tetramer has a nonreducible dimer band at 170 kDa and a monomer band at 85 kDa for 574 amino acids (68419 Da) plus 9 glycans per subunit Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 BChE
-
 Homo sapiens
3.1.1.8 butyrylcholinesterase
-
 Homo sapiens
3.1.1.8 HuBChE
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.8 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.8 0.00099
-
 pH 7.0, 25°C, recombinant enzyme Homo sapiens huprine 19

General Information

EC Number General Information Comment Organism
3.1.1.8 additional information construction of Hupresin affinity resin by coupling the huprine (inhibitor) ligand to Sepharose 4B via a 10-atom spacer between the Sepharose beads and the ligand. The spacer extends the ligand out of the Sepharose backbone, making room for the BChE protein to interact with the ligand. Hupresin compared to procainamide affinity gel for purifying BChE, method evaluation, overview. Hupresin is superior to procainamide-resin for purification of BChE, but is not suitable for purifying native acetylcholinesterase, AChE (EC 3.1.1.7), because Hupresin binds AChE so tightly that AChE is not released with buffers, but is desorbed with denaturing solvents such as 50% acetonitrile or 1% trifluoroacetic acid. Procainamide-Sepharose will continue to be useful for purification of AChE Homo sapiens
3.1.1.8 physiological function human butyrylcholinesterase (HuBChE) is an efficient bioscavenger of organophosphorus nerve agents Homo sapiens