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Literature summary extracted from

  • Mariutti, R.B.; Chaves-Moreira, D.; Vuitika, L.; Caruso, I.P.; Coronado, M.A.; Azevedo, V.A.; Murakami, M.T.; Veiga, S.S.; Arni, R.K.
    Bacterial and arachnid sphingomyelinases D comparison of biophysical and pathological activities (2017), J. Cell. Biochem., 118, 2053-2063 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 expressed in Escherichia coli BL21(DE3)RIL cells Corynebacterium pseudotuberculosis
3.1.4.41 expressed in Escherichia coli BL21(DE3)RIL cells Loxosceles intermedia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.41 0.00076
-
 sphingomyelin at pH 7.4 and 37°C Loxosceles intermedia
3.1.4.41 0.00082
-
 lysophosphatidylcholine at pH 7.4 and 37°C Loxosceles intermedia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.41 sphingomyelin + H2O Corynebacterium pseudotuberculosis
-
 ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O Loxosceles intermedia
-
 ceramide 1-phosphate + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Corynebacterium pseudotuberculosis
-
-
-
3.1.4.41 Loxosceles intermedia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.41 Ni-NTA column chromatography, Mono S column chromatography, and Superdex 75 gel filtration Corynebacterium pseudotuberculosis
3.1.4.41 Ni-NTA column chromatography, Mono S column chromatography, and Superdex 75 gel filtration Loxosceles intermedia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 lysophosphatidylcholine + H2O
-
 Corynebacterium pseudotuberculosis lysophosphatidic acid + choline
-
 ?
3.1.4.41 lysophosphatidylcholine + H2O
-
 Loxosceles intermedia lysophosphatidic acid + choline
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Corynebacterium pseudotuberculosis ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles intermedia ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O the enzyme shows a marked preference for sphingomyelin over lysophosphatidylcholine Corynebacterium pseudotuberculosis ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O the enzyme shows a marked preference for sphingomyelin over lysophosphatidylcholine Loxosceles intermedia ceramide 1-phosphate + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.41 ? x * 30000, SDS-PAGE Corynebacterium pseudotuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 RecDT1
-
 Loxosceles intermedia
3.1.4.41 SMase D
-
 Corynebacterium pseudotuberculosis
3.1.4.41 SMase D
-
 Loxosceles intermedia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.41 7.4
-
-
 Corynebacterium pseudotuberculosis
3.1.4.41 7.4
-
-
 Loxosceles intermedia