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Literature summary extracted from

  • Florez Guzman, G.Y.; Hurtado, G.B.; Ospina, S.A.
    New dextransucrase purification process of the enzyme produced by Leuconostoc mesenteroides IBUN 91.2.98 based on binding product and dextranase hydrolysis (2018), J. Biotechnol., 265, 8-14 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 Al3+ 2 mM, 99% inhibition Leuconostoc mesenteroides
2.4.1.5 Fe3+ 2 mM, 97% inhibition Leuconostoc mesenteroides
2.4.1.5 sucrose substrate inhibition at concentrations over 584 mM sucrose Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 584
-
 sucrose pH 5, 30°C Leuconostoc mesenteroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.5 extracellular
-
 Leuconostoc mesenteroides
-
-
3.2.1.11 extracellular
-
 Chaetomium erraticum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 K+ 2 mM, 27% increase in activity Leuconostoc mesenteroides
2.4.1.5 Mg2+ 2 mM, 37% increase in activity Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 170100
-
 calculation from sequence Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
-
-
2.4.1.5 Leuconostoc mesenteroides IBUN 91.2.98
-
-
-
3.2.1.11 Chaetomium erraticum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300 kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography. The obtained enzyme has a purification factor of 118 and a yield of 26% from the initial extract Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 335.1
-
 pH 5, 30°C Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose the produced dextran has a molecular size of 800-1000 kDa Leuconostoc mesenteroides D-fructose + dextran
-
 ?
2.4.1.5 sucrose the produced dextran has a molecular size of 800-1000 kDa Leuconostoc mesenteroides IBUN 91.2.98 D-fructose + dextran
-
 ?
3.2.1.11 dextran + H2O
-
 Chaetomium erraticum D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 monomer
-
 Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
3.2.1.11 Dextranase Plus L commercial preparation Chaetomium erraticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
-
 Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 3
-
 about 25% loss of activity after 20 min, about 45% loss of activity after 45 min, about 55% loss of activity after 80 min Leuconostoc mesenteroides
2.4.1.5 10
-
 about 35% loss of activity after 20 min, about 35% loss of activity after 45 min, about 40% loss of activity after 80 min Leuconostoc mesenteroides
2.4.1.5 57
-
 80 min, stable Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5
-
-
 Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.5 4 7 pH 4.0: 49% of maximal activity, pH 7.0: 25% of maximal activity Leuconostoc mesenteroides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.5 Leuconostoc mesenteroides calculation from sequence
-
 4.2