Literature summary extracted from

Haernvall, K.; Zitzenbacher, S.; Yamamoto, M.; Schick, M.B.; Ribitsch, D.; Guebitz, G.M.
A new arylesterase from Pseudomonas pseudoalcaligenes can hydrolyze ionic phthalic polyesters (2017), J. Biotechnol., 257, 70-77 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.2	expressed in Escherichia coli BL21-Gold(DE3) cells	Pseudomonas oleovorans

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.2	Pseudomonas oleovorans	A0A061D1S0	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.1.2	HisTrap column chromatography	Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.2	4-nitrophenyl butyrate + H2O	-	Pseudomonas oleovorans	4-nitrophenol + butyrate	-	?
3.1.1.2	[(3-[(3-hydroxypropoxy)carbonyl]-5-[[3-([4-[(3-hydroxypropoxy)carbonyl]benzoyl]oxy)propoxy]carbonyl]phenyl)-lambda4-sulfanetriyl]tris(oxidanide) + H2O	-	Pseudomonas oleovorans	terephthalic acid + 5-sulfoisophthalic acid + 1,3-propanediol	-	?

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
3.1.1.2	25	80	the enzyme activity towards does not significantly changed between 25°C and 80 °C	Pseudomonas oleovorans

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.1.2	7	8	-	Pseudomonas oleovorans

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Release 2023.1 (January 2023)