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Literature summary extracted from

  • Arikawa, H.; Sato, S.; Fujiki, T.; Matsumoto, K.
    A study on the relation between poly(3-hydroxybutyrate) depolymerases or oligomer hydrolases and molecular weight of polyhydroxyalkanoates accumulating in Cupriavidus necator H16 (2016), J. Biotechnol., 227, 94-102 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.22 additional information recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 (EC 3.1.1.75, UniProt ID Q0KCI0) and phaZ2 has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly Cupriavidus necator
3.1.1.75 additional information recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 and phaZ2 (EC 3.1.1.22, UniProt ID Q0K7T2) has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.22 intracellular
-
 Cupriavidus necator 5622
-
3.1.1.75 intracellular
-
 Cupriavidus necator 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Cupriavidus necator Q0K7T2 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-
3.1.1.75 Cupriavidus necator Q0KCI0 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 additional information the enzyme shows no activity with 3HB oligomers or poly(hydroxybutyrate), PHB, granules Cupriavidus necator ?
-
 ?
3.1.1.75 [(R)-3-hydroxybutanoate]n + H2O x is 1-5 Cupriavidus necator [(R)-3-hydroxybutanoate]n-x + [(R)-3-hydroxybutanoate]x
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.22 D-(-)-3-hydroxybutyrate oligomer hydrolase UniProt Cupriavidus necator
3.1.1.22 H16 A2862 locus name Cupriavidus necator
3.1.1.22 PhaZ2
-
 Cupriavidus necator
3.1.1.75 intracellular poly(3-hydroxybutyrate) depolymerase UniProt Cupriavidus necator
3.1.1.75 PhaZ1
-
 Cupriavidus necator