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Literature summary extracted from

  • Morohoshi, T.; Kamimura, Y.; Sato, N.; Iizumi, T.
    Distribution and characterization of N-acylhomoserine lactone (AHL)-degrading activity and AHL lactonase gene (qsdS) in Sphingopyxis (2018), J. Biosci. Bioeng., 127, 411-417 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis sp. EG6
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis sp. FD7
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis bauzanensis
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingomonas ursincola
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggested that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis alaskensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 heterologous expression in Escherichia coli DH5alpha Sphingopyxis sp. FD7
3.1.1.81 heterologous expression in Escherichia coli DH5alpha Sphingopyxis alaskensis
3.1.1.81 heterologous expression in Escherichia coli DH5alpha Sphingopyxis bauzanensis
3.1.1.81 heterologous expression in Escherichia coli DH5alpha Sphingomonas ursincola
3.1.1.81 heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis sp. EG6

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.81 extracellular
-
 Sphingopyxis sp. EG6
-
-
3.1.1.81 extracellular
-
 Sphingopyxis sp. FD7
-
-
3.1.1.81 extracellular
-
 Sphingomonas ursincola
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 30000
-
 SDS-PAGE Sphingopyxis sp. EG6
3.1.1.81 30000
-
 SDS-PAGE Sphingopyxis sp. FD7

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Sphingomonas ursincola
-
-
-
3.1.1.81 Sphingopyxis alaskensis
-
-
-
3.1.1.81 Sphingopyxis alaskensis DSM 13593
-
-
-
3.1.1.81 Sphingopyxis bauzanensis
-
-
-
3.1.1.81 Sphingopyxis bauzanensis DSM 22271
-
-
-
3.1.1.81 Sphingopyxis sp. EG6 A0A2Z5U8E0 isolated from industrial cooling water samples
-
3.1.1.81 Sphingopyxis sp. FD7 A0A2Z5UR29 isolated from industrial cooling water samples
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81
-
 Sphingopyxis sp. EG6
3.1.1.81 His-tagged enzyme Sphingopyxis sp. FD7
3.1.1.81 His-tagged enzyme Sphingopyxis alaskensis
3.1.1.81 His-tagged enzyme Sphingopyxis bauzanensis
3.1.1.81 His-tagged enzyme Sphingomonas ursincola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. EG6 N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. FD7 N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingomonas ursincola N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis DSM 22271 N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis DSM 13593 N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. EG6 N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. FD7 N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingomonas ursincola N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis DSM 22271 N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis DSM 13593 N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. EG6 N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. FD7 N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingomonas ursincola N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis DSM 22271 N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis DSM 13593 N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. EG6 N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. FD7 N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingomonas ursincola N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis bauzanensis DSM 22271 N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis alaskensis DSM 13593 N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. EG6 N-octanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Sphingopyxis sp. FD7 N-octanoyl-L-homoserine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.81 ? x * 30000, SDS-PAGE Sphingopyxis sp. EG6
3.1.1.81 ? x * 30000, SDS-PAGE Sphingopyxis sp. FD7

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL lactonase
-
 Sphingopyxis sp. EG6
3.1.1.81 AHL lactonase
-
 Sphingopyxis sp. FD7
3.1.1.81 AHL lactonase
-
 Sphingopyxis alaskensis
3.1.1.81 AHL lactonase
-
 Sphingopyxis bauzanensis
3.1.1.81 AHL lactonase
-
 Sphingomonas ursincola
3.1.1.81 qsdS
-
 Sphingopyxis sp. EG6
3.1.1.81 qsdS
-
 Sphingopyxis sp. FD7
3.1.1.81 qsdS
-
 Sphingopyxis alaskensis
3.1.1.81 qsdS
-
 Sphingopyxis bauzanensis
3.1.1.81 qsdS
-
 Sphingomonas ursincola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 60
-
 substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. EG6
3.1.1.81 60 70 substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. FD7

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.81 30 80 30°C: about 40% of maximal activity, 80°C: about 35% of maximal activity, substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. EG6
3.1.1.81 50 80 50°C: about 65% of maximal activity, 80°C: about 80% of maximal activity, substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. FD7

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 40
-
 5 min, stable Sphingopyxis sp. FD7
3.1.1.81 50
-
 5 min, stable Sphingopyxis sp. EG6
3.1.1.81 50
-
 5 min, 10% loss of activity Sphingopyxis sp. FD7
3.1.1.81 60
-
 5 min, 80% loss of activity Sphingopyxis sp. FD7
3.1.1.81 60
-
 5 min, about 10% loss of activity Sphingopyxis sp. EG6
3.1.1.81 70
-
 5 min, complete loss of activity Sphingopyxis sp. FD7
3.1.1.81 70
-
 5 min, about 55% loss of activity Sphingopyxis sp. EG6
3.1.1.81 80
-
 5 min, complete inactivation Sphingopyxis sp. EG6

General Information

EC Number General Information Comment Organism
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis sp. EG6
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis sp. FD7
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis alaskensis
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis bauzanensis
3.1.1.81 additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingomonas ursincola