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Literature summary extracted from

  • Chen, W.; Qu, M.; Zhou, Y.; Yang, Q.
    Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects (2018), J. Biol. Chem., 293, 2652-2660 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Pichia pastoris strain GS115 Ostrinia furnacalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14 catalytically active domains ChtII-C1 and ChtII-C2, both in unliganded form and complexed with chitooligosaccharide substrates, hanging drop vapor diffusion method, using 0.2 M sodium chloride, 0.1 M Tris, pH 8.5, and 25% (w/v) PEG3350 for ChtII-C1 and 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.5, and 45% (w/v) 2-methyl-2,4-pentadiol for ChtII-C2 Ostrinia furnacalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.00223
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtI, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 0.01193
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C2, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 0.01527
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1C2, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 0.01552
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1, at pH 6.0 and 30°C Ostrinia furnacalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Ostrinia furnacalis A0A221ZS22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 ammonium sulfate precipitation and HisTrap column chromatography Ostrinia furnacalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Ostrinia furnacalis 4-methylumbelliferol + beta-D-N,N'-diacetylchitobioside
-
 ?
3.2.1.14 alpha-chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.14 beta-chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChtII
-
 Ostrinia furnacalis
3.2.1.14 group II chitinase
-
 Ostrinia furnacalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.2
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C2, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 0.55
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 0.78
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1C2, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 1.78
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtI, at pH 6.0 and 30°C Ostrinia furnacalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 17
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C2, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 35.5
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 51
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1C2, at pH 6.0 and 30°C Ostrinia furnacalis
3.2.1.14 800
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtI, at pH 6.0 and 30°C Ostrinia furnacalis