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Literature summary extracted from

  • Nawaz, M.S.; Vik, E.S.; Berges, N.; Fladeby, C.; Bjoras, M.; Dalhus, B.; Alseth, I.
    Regulation of human endonuclease V activity and relocalization to cytoplasmic stress granules (2016), J. Biol. Chem., 291, 21786-21801 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.7 polyadenylate-binding protein C1 the association between polyadenylate-binding protein C1 and hEndoV is RNA dependent. Polyadenylate-binding protein stimulates hEndoV activity and affinity for inosine-containing RNA. Upon cellular stress, polyadenylate-binding protein C1 relocates to cytoplasmic stress granules Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.7 HEK 293T cells are transiently transfected with a vector expressing GFP-hEndoVwt Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.7 ATP 1 mM ATP almost completely inhibits hEndoV activity. hEndoV cleavage is inhibited by normal intracellular ATP concentrations. The ATP stores inside a cell do not overlay stress granules and it is suggest that hEndoV is redistributed to stress granules as a strategy to create a local environment low in ATP to permit hEndoV activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.7 cytoplasm hEndoV both have a cytoplasmic localization. It relocates to cytoplasmic stress granules in cultured human cells upon exposure to various toxic agents. This is a mechanism for the cell to regulate hEndoV activity Homo sapiens 5737
-
3.1.21.7 stress granule the enzyme relocates to cytoplasmic stress granules in cultured human cells upon exposure to various toxic agents. This is a mechanism for the cell to regulate hEndoV activity Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Homo sapiens Q8N8Q3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.7 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.7 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 RNA + H2O the enzyme cleaves RNA with inosine but not DNA Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.7 EndoV
-
 Homo sapiens