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Literature summary extracted from
- Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-beta-glucosidase identified in fungi (2015), J. Biol. Chem., 290, 1005-1019 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.104 | pharmacology | the enzyme may be a promising target for anti-cryptococcal drugs | Cryptococcus neoformans |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.104 | expressed in Escherichia coli BL21 (DE3) | Cryptococcus neoformans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.104 | 0.034 | - |
4-methylumbelliferyl beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans |  |
| 3.2.1.104 | 0.0384 | - |
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 0.0436 | - |
resorufin 3-O-beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 0.817 | - |
4-nitrophenyl beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.104 | vacuole | - |
Cryptococcus neoformans | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 120000 | - |
SDS-PAGE | Cryptococcus neoformans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.104 | Cryptococcus neoformans | A0A228BX09 | - |
- |
| 3.2.1.104 | Cryptococcus neoformans ATCC 208821 | A0A228BX09 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.104 | - |
Cryptococcus neoformans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.104 | (6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O | - |
Cryptococcus neoformans | D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol | - |
? | |
| 3.2.1.104 | (6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O | - |
Cryptococcus neoformans ATCC 208821 | D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol | - |
? | |
| 3.2.1.104 | 4-methylumbelliferyl beta-D-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + 4-methylumbelliferone | - |
? | |
| 3.2.1.104 | 4-methylumbelliferyl beta-D-glucoside + H2O | - |
Cryptococcus neoformans ATCC 208821 | D-glucose + 4-methylumbelliferone | - |
? | |
| 3.2.1.104 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + 4-nitrophenol | - |
? | |
| 3.2.1.104 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Cryptococcus neoformans ATCC 208821 | D-glucose + 4-nitrophenol | - |
? | |
| 3.2.1.104 | arbutin + H2O | - |
Cryptococcus neoformans | D-glucose + benzene-1,4-diol | - |
? | |
| 3.2.1.104 | cholesteryl 3-O-beta-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + cholesterol | - |
? | |
| 3.2.1.104 | ergosteryl 3-O-beta-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + erosterol | - |
? | |
| 3.2.1.104 | glucosylceramide + H2O | - |
Cryptococcus neoformans | D-glucose + ceramide | - |
? | |
| 3.2.1.104 | glucosylceramide + H2O | - |
Cryptococcus neoformans ATCC 208821 | D-glucose + ceramide | - |
? | |
| 3.2.1.104 | indoxyl beta-D-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + indoxyl | - |
? | |
| 3.2.1.104 | additional information | no activity with alpha-glucosides or beta-galactosides, under acidic conditions | Cryptococcus neoformans | ? | - |
? | |
| 3.2.1.104 | additional information | no activity with alpha-glucosides or beta-galactosides, under acidic conditions | Cryptococcus neoformans ATCC 208821 | ? | - |
? | |
| 3.2.1.104 | n-octyl beta-D-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + n-octanol | - |
? | |
| 3.2.1.104 | phloridzin + H2O | - |
Cryptococcus neoformans | D-glucose + phloretin | - |
? | |
| 3.2.1.104 | resorufin 3-O-beta-D-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + resorufin | - |
? | |
| 3.2.1.104 | salicin + H2O | - |
Cryptococcus neoformans | D-glucose + 2-(hydroxymethyl)phenol | - |
? | |
| 3.2.1.104 | sitosteryl 3-O-beta-glucoside + H2O | - |
Cryptococcus neoformans | D-glucose + sitosterol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.104 | ? | x * 120000, SDS-PAGE | Cryptococcus neoformans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.104 | EGCrP2 | - |
Cryptococcus neoformans |
| 3.2.1.104 | endoglycoceramidase-related protein 2 | - |
Cryptococcus neoformans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 30 | - |
assay at | Cryptococcus neoformans |
| 3.2.1.104 | 32 | 37 | - |
Cryptococcus neoformans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 16 | 40 | 16°C: about 50% of maximal activity, 40°C: about 75% of maximal activity | Cryptococcus neoformans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.104 | 0.53 | - |
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 8.8 | - |
4-methylumbelliferyl beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 13.8 | - |
4-nitrophenyl beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 21.9 | - |
resorufin 3-O-beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 5 | 5.5 | - |
Cryptococcus neoformans |
| 3.2.1.104 | 6 | - |
assay at | Cryptococcus neoformans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 4 | 7 | pH 4.0: about % of maximal activity, pH 7.0: about % of maximal activity | Cryptococcus neoformans |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.104 | malfunction | disruption of the EGCrP2 gene (egcrp2) results in the accumulation of ergosteryl 3-O-beta-glucoside, a major sterylglucoside in fungi. Distinct growth arrest, a dysfunction in cell budding, and an abnormal vacuole morphology are detected in the egcrp2-disrupted mutants | Cryptococcus neoformans |
| 3.2.1.104 | physiological function | the enzyme is involved in the catabolism of ergosteryl 3-O-beta-glucoside in the vacuoles of Cryptococcus neoformans | Cryptococcus neoformans |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.104 | 13.8 | - |
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 16.9 | - |
4-nitrophenyl beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 25.7 | - |
4-methylumbelliferyl beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
| 3.2.1.104 | 504 | - |
resorufin 3-O-beta-D-glucoside | pH 6.0, 30°C | Cryptococcus neoformans | |
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