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Literature summary extracted from

  • Kim, J.; Hannibal, L.; Gherasim, C.; Jacobsen, D.W.; Banerjee, R.
    A human vitamin B12 trafficking protein uses glutathione transferase activity for processing alkylcobalamins (2009), J. Biol. Chem., 284, 33418-33424 .
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.151 cytosol
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 Homo sapiens 5829
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.151 an alkylcobalamin + [alkylcobalamin reductase] + glutathione Homo sapiens the enzyme is involved in the cobalamin-processing pathway. It acts as a a cytosolic cobalamin trafficking chaperone cob(I)alamin-[alkylcobalamin reductase] + an S-alkylglutathione
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.151 Homo sapiens Q9Y4U1
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.151 5'-deoxyadenosylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC (a cytosolic cobalamin trafficking chaperone), directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-5'-deoxyadenosylglutathione
-
 ?
2.5.1.151 an alkylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme is involved in the cobalamin-processing pathway. It acts as a a cytosolic cobalamin trafficking chaperone Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + an S-alkylglutathione
-
 ?
2.5.1.151 butyrylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-butyrylglutathione
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 ?
2.5.1.151 ethylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-ethylglutathione
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 ?
2.5.1.151 hexylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-hexylglutathione
-
 ?
2.5.1.151 methylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-methylglutathione
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 ?
2.5.1.151 additional information in addition to its dealkylase function, the enzyme also catalyses a decyanase reaction with cyanocobalamin, cf. EC 1.16.1.6, cyanocobalamin reductase (cyanide-eliminating) Homo sapiens ?
-
 ?
2.5.1.151 pentylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-pentylglutathione
-
 ?
2.5.1.151 propylcobalamin + [alkylcobalamin reductase] + glutathione the enzyme can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. Biologically relevant thiols, e.g. cysteine and homocysteine, cannot substitute for glutathione Homo sapiens cob(I)alamin-[alkylcobalamin reductase] + S-propyglutathione
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.151 MMACHC
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 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.151 0.022
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 hexylcobalamin pH 8.0, 20°C Homo sapiens
2.5.1.151 0.024
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 pentylcobalamin pH 8.0, 20°C Homo sapiens
2.5.1.151 0.036
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 butyrylcobalamin pH 8.0, 20°C Homo sapiens
2.5.1.151 0.13
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 propylcobalamin pH 8.0, 20°C Homo sapiens
2.5.1.151 0.174
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 5'-deoxyadenosylcobalamin pH 8.0, 20°C Homo sapiens
2.5.1.151 1.86
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 ethylcobalamin pH 8.0, 20°C Homo sapiens
2.5.1.151 11.7
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 methylcobalamin pH 8.0, 20°C Homo sapiens

General Information

EC Number General Information Comment Organism
2.5.1.151 evolution the enzyme does not appear to be a sequence relative of either enzyme class and may have arisen by convergent evolution Homo sapiens
2.5.1.151 metabolism the enzyme is involved in the cobalamin-processing pathway Homo sapiens