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Literature summary extracted from

  • Lee, H.W.; Jeon, H.Y.; Choi, H.J.; Kim, N.R.; Choung, W.J.; Koo, Y.S.; Ko, D.S.; You, S.; Shim, J.H.
    Characterization and application of BiLA, a psychrophilic alpha-amylase from Bifidobacterium longum (2016), J. Agric. Food Chem., 64, 2709-2718 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.1 additional information no effect by 5 mM EDTA Bifidobacterium longum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 gene bllj_0710, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain MC1061 Bifidobacterium longum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ca2+ 60% inhibition at 5 mM Bifidobacterium longum
3.2.1.1 Cu2+ complete inhibition at 5 mM Bifidobacterium longum
3.2.1.1 Fe3+ complete inhibition at 5 mM Bifidobacterium longum
3.2.1.1 Mg2+ 65% inhibition at 5 mM Bifidobacterium longum
3.2.1.1 Mn2+ 70% inhibition at 5 mM Bifidobacterium longum
3.2.1.1 additional information no effect by 5 mM EDTA Bifidobacterium longum
3.2.1.1 Zn2+ 20% inhibition at 5 mM Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information Michaelis-Menten kinetic analysis, Km values for amylose and amylopectin of the recombinant His6-tagged enzyme are 9.2 mg/ml and 3.8 mg/ml, respectively, pH 5.0, 20°C Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Co2+ slightly activating Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bifidobacterium longum A0A0M4N1K4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant His6-tagged enzyme 88.9fold from Escherichia coli strain MC1061 by nickel affinity chromatography Bifidobacterium longum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 9.7
-
 purified recombinant His6-tagged enzyme, pH 5.0, 20°C Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 Bifidobacterium longum maltooligosaccharides
-
 ?
3.2.1.1 amylose + H2O best substrate Bifidobacterium longum maltooligosaccharides
-
 ?
3.2.1.1 cycloamylose + H2O
-
 Bifidobacterium longum maltooligosaccharides
-
 ?
3.2.1.1 glycogen + H2O
-
 Bifidobacterium longum maltooligosaccharides
-
 ?
3.2.1.1 maltoheptaose + H2O
-
 Bifidobacterium longum maltooligosaccharides
-
 ?
3.2.1.1 additional information substrate specificity, overview. Reaction product analysis by anion exchange chromatography. The enzyme reaction products are in the range of glucose to maltotetraose, whereas maltooligosaccharides smaller than maltotetraose are partially hydrolyzed. Maltose is not degraded. Maltooligosaccharides larger than maltotetraose, such as maltoheptaose, cycloamylose, and amylose, are fully hydrolyzed. Relatively small cyclodextrins, such as alpha-cyclodextrin and beta-cyclodextrin, are not hydrolyzed by enzyme BiLA, although gamma-cyclodextrin is partially hydrolyzed. Macromolecules containing alpha-1,6 linkages, such as amylopectin, starch, and glycogen, are also partially hydrolyzed to maltooligosaccharides smaller than G4. Pullulan, a polysaccharide polymer consisting of maltotriose units, is not hydrolyzed Bifidobacterium longum ?
-
 ?
3.2.1.1 raw starch + H2O from potato, corn, rice, and wheat Bifidobacterium longum maltooligosaccharides
-
 ?
3.2.1.1 soluble starch + H2O 0.5% soluble starch Bifidobacterium longum maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 48000, recombinant His6-tagged enzyme, SDS-PAGE Bifidobacterium longum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 BBG7_0117
-
 Bifidobacterium longum
3.2.1.1 BiLA
-
 Bifidobacterium longum
3.2.1.1 bllj_0710
-
 Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 20
-
-
 Bifidobacterium longum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 10 50 80% of maximal activity at 10°C, 60% at 30°C, 40% at 40°C, and 20% at 50°C, inactivation at 60°C Bifidobacterium longum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 3.4
-
 amylopectin recombinant His6-tagged enzyme, pH 5.0, 20°C Bifidobacterium longum
3.2.1.1 116.1
-
 amylose recombinant His6-tagged enzyme, pH 5.0, 20°C Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5
-
 broad optimum at pH 4.5-6.0 Bifidobacterium longum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 3 8.5 activity range, profile overview Bifidobacterium longum

General Information

EC Number General Information Comment Organism
3.2.1.1 evolution the enzyme belongs to the glycoside hydrolase family 13, GH13 Bifidobacterium longum