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Literature summary extracted from

  • Xu, Y.; Zheng, Z.; Xu, Q.; Yong, Q.; Ouyang, J.
    Efficient Conversion of Inulin to Inulooligosaccharides through Endoinulinase from Aspergillus niger (2016), J. Agric. Food Chem., 64, 2612-2618 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.7 synthesis inulooligosaccharides (IOS) represent an important class of oligosaccharides at industrial scale. Efficient conversion of inulin to IOS through endoinulinase from Aspergillus niger Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.7 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of the codon-optimized gene in Pichia pastoris strain KM71, method optimization, overview Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.7 additional information maximum activity of recombinant endoinulinase, expressed in Pichia pastoris strain KM71, is 858 U/ml obtained at 120 h of the high cell density fermentation process. Inulooligosaccharides (IOS) are harvested with high concentration of 365.1 g/l and high yield up to 91.3%. IOS with different degrees of polymerization (DP) of mainly DP3-6 are distributed in the final reaction products Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.7 inulin + H2O Aspergillus niger
-
 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O Aspergillus niger CBS 120.49
-
 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O Aspergillus niger DSM 2466
-
 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O Aspergillus niger ATCC 9029
-
 inulooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus niger A0A221C6S1
-
-
3.2.1.7 Aspergillus niger ATCC 9029 A0A221C6S1
-
-
3.2.1.7 Aspergillus niger CBS 120.49 A0A221C6S1
-
-
3.2.1.7 Aspergillus niger DSM 2466 A0A221C6S1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Aspergillus niger inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O using inulin from Jerusalem artichoke Aspergillus niger inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus niger CBS 120.49 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O using inulin from Jerusalem artichoke Aspergillus niger CBS 120.49 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus niger DSM 2466 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O using inulin from Jerusalem artichoke Aspergillus niger DSM 2466 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus niger ATCC 9029 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O using inulin from Jerusalem artichoke Aspergillus niger ATCC 9029 inulooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.7 ? x * 66200, recombinant enzyme, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 endoinulinase
-
 Aspergillus niger