EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.40 | food industry | efficient and cost-effective enzymatic production method for preparation of the high-valued natural sweetener trilobatin is developed by the combination of hydrogenation and enzymatic hydrolysis reactions with alpha-L-rhamnosidase as the catalyst in aqueous medium. This technology is adopting the cheap and largely available citrus flavanone naringin as the starting material for trilobatin synthesis, and the present enzymatic technology is possibly utilised by commercial for scale-up production. The production is a straightforward two-step process, in which naringin is hydrogenated into naringin dihydrochalcone and followed by removal of the rhamnosyl group of naringin dihydrochalcone by enzymatic hydrolysis using immobilised alpha-L-rhamnosidase as the catalyst. Under optimised conditions, an overall yield of 96% is achieved with a very low loading of alpha-L-rhamnosidase catalyst at 60 °C in a neutral aqueous buffer solution within 2 h. The immobilised alpha-L-rhamnosidase catalyst can be recycled for 10 reactions (90% yield retained) | Aspergillus niger |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.40 | enzyme immobilised onto kappa-carrageenan beads surface can be recycled for 10 reactions, 90% yield retained | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.40 | acetone | the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is ecommended to improve the hydrolysis reaction of naringin dihydrochalcone | Aspergillus niger | |
3.2.1.40 | dimethyl sulfoxide | the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is ecommended to improve the hydrolysis reaction of naringin dihydrochalcone | Aspergillus niger | |
3.2.1.40 | methanol | the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is recommended to improve the hydrolysis reaction of naringin dihydrochalcone | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.40 | Aspergillus niger | R9W5L0 | - |
- |
3.2.1.40 | Aspergillus niger JMU-TS528 | R9W5L0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.40 | naringin dihydrochalcone + H2O | - |
Aspergillus niger | trilobatin + alpha-L-rhamnose | - |
? | |
3.2.1.40 | naringin dihydrochalcone + H2O | - |
Aspergillus niger JMU-TS528 | trilobatin + alpha-L-rhamnose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 60 | - |
immobilized enzyme | Aspergillus niger |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 50 | 70 | 55°C: about 50% of maximal activity, 70°C: about 40% of maximal activity, immobilized enzyme | Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 65 | - |
significantly inactivated at the temperature above 65°C | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 5 | 6 | - |
Aspergillus niger |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 4 | 8 | when the enzyme is in the condition of pH 4.0 to 5.0 or pH 7.0 to 8.0, its activity decreases dramatically to 40% yield or even less, soluble enzyme | Aspergillus niger |