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Literature summary extracted from

  • Sudan, S.K.; Kumar, N.; Kaur, I.; Sahni, G.
    Production, purification and characterization of raw starch hydrolyzing thermostable acidic alpha-amylase from hot springs, India (2018), Int. J. Biol. Macromol., 117, 831-839 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 2-mercaptoethanol 20% inhibition at 1 mM, 30% at 5 mM Geobacillus sp. K1C
3.2.1.1 Cu2+ complete inhibition at 1 mM Geobacillus sp. K1C
3.2.1.1 Dextran complete inhibition at 1% Geobacillus sp. K1C
3.2.1.1 DTT 20% inhibition at 1 mM Geobacillus sp. K1C
3.2.1.1 EDTA over 80% inhibition at 1 mM Geobacillus sp. K1C
3.2.1.1 ethanol 20% inhibition at 10%, 40% at 20% Geobacillus sp. K1C
3.2.1.1 glycerol 20-25% inhibition at 1-2% Geobacillus sp. K1C
3.2.1.1 glycine almost complete inhibition at 1% Geobacillus sp. K1C
3.2.1.1 Hg2+ complete inhibition at 1 mM Geobacillus sp. K1C
3.2.1.1 Isoamylalcohol 70% inhibition at 10%, 90% at 20% Geobacillus sp. K1C
3.2.1.1 methanol 20% inhibition at 10%, 40% at 20% Geobacillus sp. K1C
3.2.1.1 Mg2+ 30% inhibition at 1 mM, 50% at 5 mM Geobacillus sp. K1C
3.2.1.1 Mn2+ 80% inhibition at 1-5 mM Geobacillus sp. K1C
3.2.1.1 additional information no or poor effect by 10-20% of acetone and benzol Geobacillus sp. K1C
3.2.1.1 Ni2+ no inhibition at 1 mM, 50% at 5 mM Geobacillus sp. K1C
3.2.1.1 Pb2+ 90% inhibition at 1 mM Geobacillus sp. K1C
3.2.1.1 PEG 8000 15-20% inhibition at 1-2% Geobacillus sp. K1C
3.2.1.1 PMSF 10% inhibition at 1 mM, 90% at 5 mM Geobacillus sp. K1C
3.2.1.1 Propanol 30% inhibition at 10%, 50% at 20% Geobacillus sp. K1C
3.2.1.1 SDS 40% inhibition at 0.1-0.2% Geobacillus sp. K1C
3.2.1.1 Triton X-100 15% inhibition at 0.1%, 20% at 0.2% Geobacillus sp. K1C
3.2.1.1 Tween 20 20% inhibition at 0.1-0.2% Geobacillus sp. K1C
3.2.1.1 Tween 80 10% inhibition at 0.1%, 25% at 0.2% Geobacillus sp. K1C

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 additional information the activity of the purified enzyme is Ca2+-independent Geobacillus sp. K1C

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 59000
-
 about, gel filtration Geobacillus sp. K1C

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Geobacillus sp. K1C
-
 isolated from Manikaran hot springs, India
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 native extracellular enzyme 49fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration Geobacillus sp. K1C

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 26493
-
 purified native enzyme, pH 6.0, 80°C, substrate soluble starch Geobacillus sp. K1C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 additional information substrate specificity, overview Geobacillus sp. K1C ?
-
 ?
3.2.1.1 raw corn starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
3.2.1.1 raw potato starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
3.2.1.1 raw rice starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
3.2.1.1 raw wheat starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
3.2.1.1 soluble starch + H2O 0.5% starch solution Geobacillus sp. K1C ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 59000, SDS-PAGE Geobacillus sp. K1C
3.2.1.1 More presence of random coil conformation Geobacillus sp. K1C

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 80
-
-
 Geobacillus sp. K1C

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 60 95 over 50% of maximal activity within this range, profile overview Geobacillus sp. K1C

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 additional information
-
 in the presence of substrate, thermostability of the alpha-amylase is increased Geobacillus sp. K1C
3.2.1.1 70
-
 purified native enzyme, 40% activity is retained after 2 h, 20% after 5 h in absence of substrate, while in presence of 0.1% starch substrate, over 80% activity is retained for 8 h Geobacillus sp. K1C
3.2.1.1 80
-
 purified native enzyme, 15% activity is retained after 1 h, 10% after 2 h in absence of substrate, while in presence of 0.1% starch substrate, about 30% activity is retained for 4-8 h, 20% remaining after 9 h Geobacillus sp. K1C

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6
-
-
 Geobacillus sp. K1C

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 4 10 activity range, profile overview Geobacillus sp. K1C

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5 7 purified native enzyme, 90% initial activity is retained after 48 h Geobacillus sp. K1C