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Literature summary extracted from

  • Du, R.; Song, Q.; Zhang, Q.; Zhao, F.; Kim, R.C.; Zhou, Z.; Han, Y.
    Purification and characterization of novel thermostable and Ca-independent alpha-amylase produced by Bacillus amyloliquefaciens BH072 (2018), Int. J. Biol. Macromol., 115, 1151-1156 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.1 2-mercaptoethanol 9-12% activation at 1-5% Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Cu2+ 25% inhibition at 5 mM Bacillus amyloliquefaciens
3.2.1.1 EDTA
-
 Bacillus amyloliquefaciens
3.2.1.1 Fe2+ 35% inhibition at 5 mM Bacillus amyloliquefaciens
3.2.1.1 Hg2+ complete inhibition at 5 mM Bacillus amyloliquefaciens
3.2.1.1 additional information no or poor inhibition by Ba2+, K+, Ca2+, Mn2+, and Co2+ at 5-50 mM and by SDS, PMSF, Triton X-100, and Tween 20 at 1-5% Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information Km of BH072 alpha-amylase is 4.27 mg/ml Bacillus amyloliquefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular the enzyme is secreted Bacillus amyloliquefaciens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Mg2+ 38% activation at 5 mM Bacillus amyloliquefaciens
3.2.1.1 additional information BH072 alpha-amylase is a Ca2+-independent enzyme Bacillus amyloliquefaciens
3.2.1.1 Zn2+ 22% activation at 5 mM Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus amyloliquefaciens
-
 isolated from honey
-
3.2.1.1 Bacillus amyloliquefaciens BH072
-
 isolated from honey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 native enzyme from strain BH072 21.23fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 2162.42
-
 purified native enzyme, pH 7.0, 60°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 raw starch + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.2.1.1 raw starch + H2O
-
 Bacillus amyloliquefaciens BH072 ?
-
 ?
3.2.1.1 soluble starch + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.2.1.1 soluble starch + H2O
-
 Bacillus amyloliquefaciens BH072 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 BH072alpha-amylase
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 60
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 30 90 55% of maximal activity at 30°C, 80% at 80°C, and 30% at 90°C, profile overview Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 50
-
 purified enzyme, 70% activity remaining after 42 h Bacillus amyloliquefaciens
3.2.1.1 60
-
 purified enzyme, 95% activity remaining after 42 h Bacillus amyloliquefaciens
3.2.1.1 70
-
 purified enzyme, 80% activity remaining after 42 h Bacillus amyloliquefaciens
3.2.1.1 80
-
 purified enzyme, 75% activity remaining after 42 h Bacillus amyloliquefaciens
3.2.1.1 85
-
 purified enzyme, 25% activity remaining after 42 h Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 7
-
-
 Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5.5
-
 purified enzyme, 20% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 6
-
 purified enzyme, 80% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 6.5
-
 purified enzyme, 90% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 7
-
 purified enzyme, 95% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 7.5
-
 purified enzyme, 90% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 8
-
 purified enzyme, 80% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 8.5
-
 purified enzyme, 50% activity remaining after 200 min Bacillus amyloliquefaciens
3.2.1.1 9
-
 purified enzyme, 40% activity remaining after 200 min Bacillus amyloliquefaciens

General Information

EC Number General Information Comment Organism
3.2.1.1 additional information N-terminal amino acid sequencing using mass spectrometry for analysis and homology modelling of the catalytic domain, overview Bacillus amyloliquefaciens