Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kahar, U.M.; Chan, K.G.; Sani, M.H.; Mohd Noh, N.I.; Goh, K.M.
    Effects of single and co-immobilization on the product specificity of type I pullulanase from Anoxybacillus sp. SK3-4 (2017), Int. J. Biol. Macromol., 104, 322-332 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli BL21(DE3) Anoxybacillus ayderensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Anoxybacillus ayderensis EPZ37738
-
-
3.2.1.41 Anoxybacillus ayderensis DSM 28779 EPZ37738
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Anoxybacillus ayderensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 pullulan + H2O soluble enzyme is highly reactive towards pullulan but was unable to degrade short branches in starch Anoxybacillus ayderensis maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O soluble enzyme is highly reactive towards pullulan but was unable to degrade short branches in starch Anoxybacillus ayderensis DSM 28779 maltotriose + ?
-
 ?
3.2.1.41 starch + H2O following immobilization through covalent attachment to three epoxides (ReliZyme EP403/M, ImmobeadIB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M), all PulASK derivatives are active on both short and long branches in starch producing reducing sugars (predominantly maltotriose) and oligosaccharides (chain lenght G8 and higher), respectively, a feature that is absent in the free enzyme. Individual or coimmobilization causes changes in the product specificity, presumably due to changes in the enzyme binding pocket caused by the influence of carrier surface properties (hydrophobicor hydrophilic) and the lengths of the spacer arms Anoxybacillus ayderensis maltotriose + oligosaccharides
-
 ?
3.2.1.41 starch + H2O following immobilization through covalent attachment to three epoxides (ReliZyme EP403/M, ImmobeadIB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M), all PulASK derivatives are active on both short and long branches in starch producing reducing sugars (predominantly maltotriose) and oligosaccharides (chain lenght G8 and higher), respectively, a feature that is absent in the free enzyme. Individual or coimmobilization causes changes in the product specificity, presumably due to changes in the enzyme binding pocket caused by the influence of carrier surface properties (hydrophobicor hydrophilic) and the lengths of the spacer arms Anoxybacillus ayderensis DSM 28779 maltotriose + oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 PulASK
-
 Anoxybacillus ayderensis