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Literature summary extracted from

  • Juarez-Hernandez, E.O.; Casados-Vazquez, L.E.; Bideshi, D.K.; Salcedo-Hernandez, R.; Barboza-Corona, J.E.
    Role of the C-terminal and chitin insertion domains on enzymatic activity of endochitinase ChiA74 of Bacillus thuringiensis (2017), Int. J. Biol. Macromol., 102, 52-59 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Escherichia coli Rosetta 2 cells Bacillus thuringiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ca2+ about 30% residual activity at 1 mM Bacillus thuringiensis
3.2.1.14 Mg2+ about 30% residual activity at 1 mM Bacillus thuringiensis
3.2.1.14 Mn2+ about 30% residual activity at 1 mM Bacillus thuringiensis
3.2.1.14 Zn2+ about 30% residual activity at 1 mM Bacillus thuringiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 2.99
-
 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis
3.2.1.14 9.66
-
 colloidal chitin mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 colloidal chitin + H2O Bacillus thuringiensis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus thuringiensis Q93AD3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 HiTrap Ni affinity column chromatography Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Bacillus thuringiensis ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Bacillus thuringiensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 70000, mature enzyme lacking its signal peptide sequence, SDS-PAGE Bacillus thuringiensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA74
-
 Bacillus thuringiensis
3.2.1.14 endochitinase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40
-
-
 Bacillus thuringiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 4.24
-
 colloidal chitin mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis
3.2.1.14 4.98
-
 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7
-
-
 Bacillus thuringiensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 0.43
-
 colloidal chitin mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis
3.2.1.14 1.66
-
 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis