Any feedback?
Literature summary extracted from
- Achromobacter denitrificans strain SP1 produces an intracellular esterase upon utilizing di(2ethylhexyl)phthalate (2015), Int. Biodeter. Biodegrad., 105, 160-167 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.60 | synthesis | optimum conditions for the production of the maximum (30.5 U) intracellular esterase, are 10 mM di(2-ethylhexyl)phthalate and 72 h incubation at pH 8.0 were found as optimum conditions | Achromobacter denitrificans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.60 | 1.308 | - |
4-nitrophenyl acetate | 50°C, pH 8.0 | Achromobacter denitrificans |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.60 | Mn2+ | 0.25 mM, maximum esterase activity | Achromobacter denitrificans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.60 | Achromobacter denitrificans | - |
- |
- |
| 3.1.1.60 | Achromobacter denitrificans SP1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.60 | 89.5 | - |
substrate 4-nitrophenyl acetate, pH 50°C, pH 8.0 | Achromobacter denitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.60 | 4-nitrophenyl acetate + H2O | - |
Achromobacter denitrificans | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.60 | 4-nitrophenyl acetate + H2O | - |
Achromobacter denitrificans SP1 | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.60 | di(2-ethylhexyl)phthalate + H2O | - |
Achromobacter denitrificans | 2-ethylhexyl phthalate + 2-ethylhexan-1-ol | - |
? | |
| 3.1.1.60 | di(2-ethylhexyl)phthalate + H2O | - |
Achromobacter denitrificans SP1 | 2-ethylhexyl phthalate + 2-ethylhexan-1-ol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.60 | ? | x * 53000, SDS-PAGE | Achromobacter denitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.60 | 50 | - |
- |
Achromobacter denitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.60 | 8 | - |
- |
Achromobacter denitrificans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
