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Literature summary extracted from
- Ergosteryl-beta-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae (2015), Glycobiology, 25, 1079-1089 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
| 3.2.1.104 | expressed in Escherichia coli BL21 (DE3) | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.104 | cytosol | the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h) | Saccharomyces cerevisiae | 5829 | - |
| 3.2.1.104 | membrane | the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h) | Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 138000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | Saccharomyces cerevisiae | - |
- |
- |
| 3.2.1.62 | Saccharomyces cerevisiae BY4741 | - |
- |
- |
| 3.2.1.104 | Saccharomyces cerevisiae | P40566 | - |
- |
| 3.2.1.104 | Saccharomyces cerevisiae ATCC 204508 | P40566 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | nickel-conjugated Sepharose 6 column chromatography and Superdex 200 gel filtration | Saccharomyces cerevisiae |
| 3.2.1.104 | - |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 4-methylumberifellyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | 4-methylumbelliferone + beta-D-glucose | - |
? |  |
| 3.2.1.62 | 4-nitrophenyl alpha-D-galactoside + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl alpha-D-glucoside + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-arabinopyranoside + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + beta-D-arabinopyranose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-fucoside + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-fucoside + H2O | very low activity | Saccharomyces cerevisiae BY4741 | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-galactoside + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-galactoside + H2O | very low activity | Saccharomyces cerevisiae BY4741 | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-GlcNAc + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + beta-D-GlcNAc | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae BY4741 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-xyloside + H2O | very low activity | Saccharomyces cerevisiae | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.62 | C6-7-nitro-2,1,3-benzoxadiazole-GlcCer | - |
Saccharomyces cerevisiae | C6-7-nitro-2,1,3-benzoxadiazole-ceramide + beta-D-glucose | - |
? | |
| 3.2.1.62 | cholesteryl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | cholesterol + beta-D-glucose | - |
? | |
| 3.2.1.62 | ergosteryl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | ergosterol + beta-D-glucose | - |
? | |
| 3.2.1.62 | glucosylceramide + H2O | - |
Saccharomyces cerevisiae | beta-D-glucose + ceramide | - |
? | |
| 3.2.1.62 | glucosylceramide + H2O | - |
Saccharomyces cerevisiae BY4741 | beta-D-glucose + ceramide | - |
? | |
| 3.2.1.62 | additional information | no activity with C6-7-nitro-2,1,3-benzoxadiazole-sphingomyelin, 4-nitrophenyl alpha-D-fucoside, 4-nitrophenyl alpha-D-GalNAc, 4-nitrophenyl beta-D-GalNAc, 4-nitrophenyl alpha-D-GlcNAc, 4-nitrophenyl alpha-D-mannoside, 4-nitrophenyl beta-D-mannoside, alpha-D-xylose, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl alpha-D-rhamnoside, 4-nitrophenyl alpha-D-sialoside, and 4-nitrophenyl beta-D-GlcUA | Saccharomyces cerevisiae | ? | - |
? | |
| 3.2.1.62 | additional information | no activity with C6-7-nitro-2,1,3-benzoxadiazole-sphingomyelin, 4-nitrophenyl alpha-D-fucoside, 4-nitrophenyl alpha-D-GalNAc, 4-nitrophenyl beta-D-GalNAc, 4-nitrophenyl alpha-D-GlcNAc, 4-nitrophenyl alpha-D-mannoside, 4-nitrophenyl beta-D-mannoside, alpha-D-xylose, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl alpha-D-rhamnoside, 4-nitrophenyl alpha-D-sialoside, and 4-nitrophenyl beta-D-GlcUA | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
| 3.2.1.62 | sitosteryl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | sitosterol + beta-D-glucose | - |
? | |
| 3.2.1.104 | 4-methylumbelliferyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + 4-methylumbelliferone | - |
? | |
| 3.2.1.104 | 4-methylumbelliferyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + 4-methylumbelliferone | - |
? | |
| 3.2.1.104 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + 4-nitrophenol | - |
? | |
| 3.2.1.104 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + 4-nitrophenol | - |
? | |
| 3.2.1.104 | cholesteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + cholesterol | - |
? | |
| 3.2.1.104 | cholesteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + cholesterol | - |
? | |
| 3.2.1.104 | ergosteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + ergosterol | - |
? | |
| 3.2.1.104 | ergosteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + ergosterol | - |
? | |
| 3.2.1.104 | glucosylceramide + H2O | - |
Saccharomyces cerevisiae | D-glucose + ceramide | - |
? | |
| 3.2.1.104 | glucosylceramide + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + ceramide | - |
? | |
| 3.2.1.104 | sitosteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + sitosterol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | ? | x * 138000, SDS-PAGE | Saccharomyces cerevisiae |
| 3.2.1.104 | ? | x * 138000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | Egh1 | - |
Saccharomyces cerevisiae |
| 3.2.1.62 | ergosteryl-beta-glucosidase | - |
Saccharomyces cerevisiae |
| 3.2.1.62 | Yir007w | - |
Saccharomyces cerevisiae |
| 3.2.1.104 | Egh1 | - |
Saccharomyces cerevisiae |
| 3.2.1.104 | ergosteryl-beta-glucosidase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.104 | 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 5 | - |
- |
Saccharomyces cerevisiae |
| 3.2.1.104 | 5.5 | - |
assay at | Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 4.5 | 6.5 | about 90% activity at pH 4.5, 100% activity at pH 5.0, about 70% activity at pH 5.5, about 60% activity at pH 6.0, about 50% activity at pH 6.5, about 20% activity at pH 7.0, no activity at pH 8.5 | Saccharomyces cerevisiae |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | malfunction | enzyme gene disruption results in the accumulation of ergosteryl beta-D-glucoside and fragmentation of vacuoles | Saccharomyces cerevisiae |
| 3.2.1.62 | physiological function | the enzyme is involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
| 3.2.1.104 | malfunction | the disruption of EGH1 in Saccharomyces cerevisiae BY4741 (egh1DELTA) results in the accumulation of ergosteryl 3-O-beta-glucoside and fragmentation of vacuoles | Saccharomyces cerevisiae |
| 3.2.1.104 | physiological function | involved in sterylglucoside catabolism and vacuole formation | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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