Literature summary extracted from
Kotani, S.; Izawa, S.; Komai, N.; Takayanagi, A.; Arioka, M.
Mitochondria-localized phospholipase A2, AoPlaA, in Aspergillus oryzae displays phosphatidylethanolamine-specific activity and is involved in the maintenance of mitochondrial phospholipid composition (2016), Fungal Genet. Biol., 96, 1-11 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.4 |
expression in Escherichia coli |
Aspergillus oryzae |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.1.4 |
R238A |
the activity of the R238A mutant isx0140% of that of wild type enzyme. The growth of the strain overexpressing R238A is similar to that of the strain overexpressing wild type AoPlaA, presumably because the residual activity of R238A is sufficient to cause growth retardation |
Aspergillus oryzae |
3.1.1.4 |
S266A |
mutation abolishes the enzyme activity |
Aspergillus oryzae |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.1.1.4 |
mitochondrion |
- |
Aspergillus oryzae |
5739 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.1.4 |
Ca2+ |
free Ca2+ enhances the activity of mAoPlaA, but even without the added Ca2+ or in the presence of EGTA to chelate free Ca2+, mAoPlaA displays more than 20% of maximum activity |
Aspergillus oryzae |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.4 |
Aspergillus oryzae |
C7G4B6 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.1.4 |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.1.4 |
1-palmitoyl-2-oleoyl-phosphatidylethanolamine + H2O |
phosphatidylethanolamine-specific activity. The enzyme displays phospholipases A2 activity to phosphatidylethanolamine, but not to other phospholipids and generates 1-acylated lyso-phosphatidylethanolamine |
Aspergillus oryzae |
1-palmitoyl-lysophosphatidylethanolamine + oleate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.4 |
mAoPlaA |
- |
Aspergillus oryzae |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.1.1.4 |
malfunction |
the AoplaA-disruption strain displays increased content of cardiolipin |
Aspergillus oryzae |
3.1.1.4 |
metabolism |
the enzyme is involved in the maintenance of mitochondrial phospholipid composition |
Aspergillus oryzae |