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Literature summary extracted from
- Production of chimeric acidic alpha-amylase by the recombinant Pichia pastoris and its applications (2017), Front. Microbiol., 8, 493 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | recombinant expression of chimeric acidic alpha-amylase Ba-Gt-amy in Pichia pastoris strain X33, real-time RT-PCR enzyme expression analysis | Geobacillus thermoleovorans |
| 3.2.1.1 | recombinant expression of chimeric acidic alpha-amylase Ba-Gt-amy in Pichia pastoris strain X33, real-time RT-PCR enzyme expression analysis | Bacillus acidicola |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | additional information | construction of the chimeric amylase Ba-Gt-amy having catalytic domain from acidic amylase of Bacillus acidicola and N- and C-terminal additional amino acids from thermophilic alpha-amylase of Geobacillus thermoleovorans. Generation of multi-copy integrants that can secrete high levels of heterologous protein using multiple transformation approach followed by post-transformational vector amplification (PTVA). Method evaluation and optimization | Geobacillus thermoleovorans |
| 3.2.1.1 | additional information | construction of the chimeric amylase Ba-Gt-amy having catalytic domain from acidic amylase of Bacillus acidicola and N- and C-terminal additional amino acids from thermophilic alpha-amylase of Geobacillus thermoleovorans. Generation of multi-copy integrants that can secrete high levels of heterologous protein using multiple transformation approach followed by post-transformational vector amplification (PTVA). Method evaluation and optimization | Bacillus acidicola |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | Km for raw starch is 0.79 mg/ml | Geobacillus thermoleovorans | |
| 3.2.1.1 | additional information | - |
additional information | Km for raw starch is 0.79 mg/ml | Bacillus acidicola |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Bacillus acidicola | - |
- |
- |
| 3.2.1.1 | Geobacillus thermoleovorans | J9PXA2 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | glycoprotein | recombinant chimeric amylase Ba-Gt-amy is glycosylated, it has 6 N- and 6 O-glycosylation sites | Geobacillus thermoleovorans |
| 3.2.1.1 | glycoprotein | recombinant chimeric amylase Ba-Gt-amy is glycosylated, it has 6 N- and 6 O-glycosylation sites | Bacillus acidicola |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | raw starch + H2O | - |
Geobacillus thermoleovorans | ? | - |
? |  |
| 3.2.1.1 | raw starch + H2O | - |
Bacillus acidicola | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | acidic amylase | - |
Geobacillus thermoleovorans |
| 3.2.1.1 | acidic amylase | - |
Bacillus acidicola |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 60 | - |
recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Geobacillus thermoleovorans |
| 3.2.1.1 | 60 | - |
recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Bacillus acidicola |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 70 | - |
purified recombinant chimeric acidic alpha-amylase Ba-Gt-amy, T1/2 is 40 min | Geobacillus thermoleovorans |
| 3.2.1.1 | 70 | - |
purified recombinant chimeric acidic alpha-amylase Ba-Gt-amy, T1/2 is 40 min | Bacillus acidicola |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | 40000 | - |
raw starch | pH 4.0, 60°C, recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Geobacillus thermoleovorans | |
| 3.2.1.1 | 40000 | - |
raw starch | pH 4.0, 60°C, recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Bacillus acidicola |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 4 | - |
recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Geobacillus thermoleovorans |
| 3.2.1.1 | 4 | - |
recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Bacillus acidicola |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 3 | 6 | activity range | Geobacillus thermoleovorans |
| 3.2.1.1 | 3 | 6 | activity range | Bacillus acidicola |
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