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Literature summary extracted from

  • Lu, X.; Lin, J.; Wang, C.; Du, X.; Cai, J.
    Purification and characterization of exo-polygalacturonase from Zygoascus hellenicus V25 and its potential application in fruit juice clarification (2016), Food Sci. Biotechnol., 25, 1379-1385 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.67 2-mercaptoethanol 116.31% activity at 5 mM Zygoascus hellenicus
3.2.1.67 L-cysteine 121.4% activity at 5 mM Zygoascus hellenicus
3.2.1.67 phenylmethylsulfonyl fluoride 113.77% activity at 5 mM Zygoascus hellenicus
3.2.1.67 Tween 20 109.11% activity at 5 mM Zygoascus hellenicus
3.2.1.67 Tween 80 115.4% activity at 5 mM Zygoascus hellenicus
3.2.1.67 Urea 110.38% activity at 5 mM Zygoascus hellenicus

Application

EC Number Application Comment Organism
3.2.1.67 food industry the enzyme is applied in the juice clarification of tangerine, orange, grapefruit, and apple Zygoascus hellenicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Al3+ 84.27% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 Ba2+ 85.24% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 Co2+ 87.23% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 Fe2+ 90.07% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 GSH 83.26% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 Hg2+ 31.41% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 Pb2+ 87.53% residual activity at 10 mM Zygoascus hellenicus
3.2.1.67 phenylmethylsulfonyl fluoride 81.4% residual activity at 10 mM Zygoascus hellenicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Zygoascus hellenicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Cu2+ 140.11% activity at 10 mM Zygoascus hellenicus
3.2.1.67 K+ 117.79% activity at 10 mM Zygoascus hellenicus
3.2.1.67 Mg2+ 113.53% activity at 10 mM Zygoascus hellenicus
3.2.1.67 Na+ 108.74% activity at 10 mM Zygoascus hellenicus
3.2.1.67 Zn2+ 112.44% activity at 10 mM Zygoascus hellenicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Zygoascus hellenicus
-
-
-
3.2.1.67 Zygoascus hellenicus V25
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 ammonium sulfate precipitation, DEAE cellulose chromatography, and Sephadex G-100 gel filtration Zygoascus hellenicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 citrus pectin DE 30% + H2O 100% activity Zygoascus hellenicus galacturonic acid
-
 ?
3.2.1.67 citrus pectin DE 30% + H2O 100% activity Zygoascus hellenicus V25 galacturonic acid
-
 ?
3.2.1.67 citrus pectin DE 72% + H2O 58.19% activity compared to citrus pectin DE 30% Zygoascus hellenicus galacturonic acid
-
 ?
3.2.1.67 citrus pectin DE 72% + H2O 58.19% activity compared to citrus pectin DE 30% Zygoascus hellenicus V25 galacturonic acid
-
 ?
3.2.1.67 citrus pectin DE 85% + H2O 7.18% activity compared to citrus pectin DE 30% Zygoascus hellenicus galacturonic acid
-
 ?
3.2.1.67 citrus pectin DE 85% + H2O 7.18% activity compared to citrus pectin DE 30% Zygoascus hellenicus V25 galacturonic acid
-
 ?
3.2.1.67 additional information no activity with xylan and carboxymethyl cellulose Zygoascus hellenicus ?
-
 ?
3.2.1.67 additional information no activity with xylan and carboxymethyl cellulose Zygoascus hellenicus V25 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 75280, calculated from amino acid sequence Zygoascus hellenicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-polygalacturonanase
-
 Zygoascus hellenicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 60
-
-
 Zygoascus hellenicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 35 70 the enzyme shows more than 50% activity between 35 and 70°C Zygoascus hellenicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 30 50 the purified enzyme is stable between 30 and 50°C, retaining more than 90% of its activity after 4 h, but the thermal stability of the enzyme obviously declines at 60-70°C Zygoascus hellenicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 5
-
-
 Zygoascus hellenicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 3 7 the enzyme shows more than 70% activity at pH 3.0-7.0 Zygoascus hellenicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 3 11 the enzyme retains more than 85% of its activity at pH 3.0-11.0 in 4 h Zygoascus hellenicus