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Literature summary extracted from

  • Zhang, T.; Yuan, W.; Li, M.; Miao, M.; Mu, W.
    Purification and characterization of an intracellular alpha-L-rhamnosidase from a newly isolated strain, Alternaria alternata SK37.001 (2018), Food Chem., 269, 63-69 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.40 food industry the enzyme is used to enhance wine aromas or to debitter citrus juices by releasing L-rhamnose Alternaria alternata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 Co2+ 2 mM, 36% loss of activity Alternaria alternata
3.2.1.40 Cu2+ 2 mM, 53% loss of activity Alternaria alternata
3.2.1.40 Mg2+ 2 mM, slight inhibition Alternaria alternata
3.2.1.40 Mn2+ 2 mM, slight inhibition Alternaria alternata
3.2.1.40 Ni2+ 2 mM, complete loss of activity Alternaria alternata
3.2.1.40 Zn2+ 2 mM, slight inhibition Alternaria alternata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 4.16
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.5, 50°C Alternaria alternata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.40 intracellular
-
 Alternaria alternata 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.40 Al3+ 2 mM, 1.25fold activation Alternaria alternata
3.2.1.40 Ba2+ 2 mM, 1.35fold activation Alternaria alternata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 66000
-
 SDS-PAGE Alternaria alternata
3.2.1.40 135000
-
 gel filtration Alternaria alternata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Alternaria alternata
-
-
-
3.2.1.40 Alternaria alternata SK37.001
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Alternaria alternata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.40 mycelium
-
 Alternaria alternata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 21.7
-
 purified enzyme, pH 5.5, 50°C Alternaria alternata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Alternaria alternata 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Alternaria alternata SK37.001 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Alternaria alternata hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 additional information the enzyme can not hydrolyze ginsenoside Rg2 or saikosaponin C Alternaria alternata ?
-
 ?
3.2.1.40 additional information the enzyme can not hydrolyze ginsenoside Rg2 or saikosaponin C Alternaria alternata SK37.001 ?
-
 ?
3.2.1.40 naringin + H2O
-
 Alternaria alternata beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Alternaria alternata SK37.001 beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 neohesperidin + H2O
-
 Alternaria alternata 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?
3.2.1.40 neohesperidin + H2O
-
 Alternaria alternata SK37.001 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?
3.2.1.40 quercitrin + H2O
-
 Alternaria alternata quercetin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Alternaria alternata isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Alternaria alternata SK37.001 isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 dimer 1 * 66000, SDS-PAGE Alternaria alternata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 60
-
-
 Alternaria alternata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 40 70 40°C: about 55% of maximal activity, 70°C: about 30% of maximal activity Alternaria alternata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 30 50 4 h, residual enzyme activity is more than 60% Alternaria alternata
3.2.1.40 50
-
 t1/2: 630 min Alternaria alternata
3.2.1.40 55
-
 t1/2: 240 min Alternaria alternata
3.2.1.40 60
-
 t1/2: 59.5 min Alternaria alternata
3.2.1.40 65
-
 t1/2: 45.9 min Alternaria alternata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 5.5
-
 substrate: 4-nitrophenyl alpha-L-rhamnopyranoside Alternaria alternata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 4 8 12 h, 4°C, most of its activity is retained Alternaria alternata