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Literature summary extracted from

  • Sagu, S.T.; Nso, E.J.; Homann, T.; Kapseu, C.; Rawel, H.M.
    Extraction and purification of beta-amylase from stems of Abrus precatorius by three phase partitioning (2015), Food Chem., 183, 144-153 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.2 industry amylases are important industrial enzymes used in starch liquefaction to produce glucose, fructose and maltose and also in brewing, baking, textile, paper, detergent and sugar industries Abrus precatorius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.2 additional information
-
 additional information Km and Vmax values are 79.37mg/ml and 5.13 U/ml, respectively, at 50°C, pH 6.0, Michaelis-Menten kinetics Abrus precatorius

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Abrus precatorius
-
 harvested in Ngaoundere, Adamawa, region in Northern Cameroon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2 native enzyme enriched fraction from powdered stems of Abrus precatorius using a three-phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulfate saturation and pH). Data fitting in a second-order polynomial model and parameters optimization to enrich beta-amylase. Optimal conditions are: a ratio of crude extract/tert-butanol of 0.87 v/v, saturation in ammonium sulfate of 49.46% w/v, and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 are found. Next steps are dialysis and lyophilisation Abrus precatorius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.2 stem
-
 Abrus precatorius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.2 3.79
-
 crude enzyme extract, pH 6.0, 50°C Abrus precatorius
3.2.1.2 19.99
-
 TPP-interfacial precipitate enzyme after dialysis and lyophilisation, pH 6.0, 50°C Abrus precatorius
3.2.1.2 24.59
-
 TPP-interfacial precipitate enzyme after dialysis, pH 6.0, 50°C Abrus precatorius
3.2.1.2 38.24
-
 TPP-interfacial precipitate enzyme, pH 6.0, 50°C Abrus precatorius

Subunits

EC Number Subunits Comment Organism
3.2.1.2 ? x * 60100, SDS-PAGE Abrus precatorius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 70
-
 native enzyme Abrus precatorius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.2 65
-
 purified native enzyme, pH 6.0-6.5, stable up to Abrus precatorius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 6 6.5 native enzyme Abrus precatorius