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Literature summary extracted from

  • Lansky, S.; Zehavi, A.; Belrhali, H.; Shoham, Y.; Shoham, G.
    Structural basis for enzyme bifunctionality - the case of Gan1D from Geobacillus stearothermophilus (2017), FEBS J., 284, 3931-3953 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.85 in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5 Geobacillus stearothermophilus
3.2.1.86 in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5 Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.85 E170Q catalytic mutant Geobacillus stearothermophilus
3.2.1.85 W433A the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity Geobacillus stearothermophilus
3.2.1.85 W433M the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity Geobacillus stearothermophilus
3.2.1.86 E170Q catalytic mutant Geobacillus stearothermophilus
3.2.1.86 W433A the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity Geobacillus stearothermophilus
3.2.1.86 W433M the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.85 115000
-
 gel filtration Geobacillus stearothermophilus
3.2.1.86 115000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Geobacillus stearothermophilus W8QF82
-
-
3.2.1.85 Geobacillus stearothermophilus T-1 W8QF82
-
-
3.2.1.86 Geobacillus stearothermophilus W8QF82
-
-
3.2.1.86 Geobacillus stearothermophilus T-1 W8QF82
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85
-
 Geobacillus stearothermophilus
3.2.1.86
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 cellobiose-6-phosphate + H2O
-
 Geobacillus stearothermophilus D-glucose-6-phosphate + D-glucose
-
 ?
3.2.1.85 cellobiose-6-phosphate + H2O
-
 Geobacillus stearothermophilus T-1 D-glucose-6-phosphate + D-glucose
-
 ?
3.2.1.86 lactose 6-phosphate + H2O
-
 Geobacillus stearothermophilus beta-D-galactose-6-phosphate + D-glucose
-
 ?
3.2.1.86 lactose 6-phosphate + H2O
-
 Geobacillus stearothermophilus T-1 beta-D-galactose-6-phosphate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.85 homodimer 2 * 55204, calculated from amino acid sequence Geobacillus stearothermophilus
3.2.1.86 homodimer 2 * 55204, calculated from amino acid sequence Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 Gan1D the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities Geobacillus stearothermophilus
3.2.1.86 Gan1D the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities Geobacillus stearothermophilus

Expression

EC Number Organism Comment Expression
3.2.1.85 Geobacillus stearothermophilus the enzyme expression is upregulated by D-galactose up
3.2.1.86 Geobacillus stearothermophilus the enzyme expression is upregulated by D-galactose up