EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.7 | additional information | usage of CRISPR/Cas9-mediated genome editing to generate a loss-of-function bpnt-1 allele, qd303, in which a deletion/insertion leads to a frameshift at amino acid 20 followed by a premature stop codon. bpnt-1 mRNA is almost entirely absent in the bpnt-1(qd303) mutant, demonstrating that the bpnt-1 expression pattern is specific. Animals carrying bpnt-1(qd303), or the W294X nonsense mutation bpnt-1(gk469190), fail to express daf-7 in the ASJ neurons when exposed to Pseudomonas aeruginosa. Notably, the constitutive expression of daf-7 in the ASI neuron pair is unaffected by loss of bpnt-1. BPNT-1 is required for the expression of daf-7 specifically in the ASJ neurons | Caenorhabditis elegans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.7 | Li+ | a lithium-sensitive enzyme, the ASJ neurons are selectively and reversibly inhibited by lithium, molecular mechanism by which loss of BPNT-1 leads to dysfunction of the ASJ neuron, overview | Caenorhabditis elegans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.7 | cytosol | - |
Caenorhabditis elegans | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.7 | adenosine 3',5'-bisphosphate + H2O | Caenorhabditis elegans | - |
AMP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.7 | Caenorhabditis elegans | Q23493 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.7 | brain | - |
Caenorhabditis elegans | - |
3.1.3.7 | neuron | ASJ neurons | Caenorhabditis elegans | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.7 | adenosine 3',5'-bisphosphate + H2O | - |
Caenorhabditis elegans | AMP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.7 | bisphosphate 3'-nucleotidase | - |
Caenorhabditis elegans |
3.1.3.7 | BPNT-1 | - |
Caenorhabditis elegans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.7 | evolution | BPNT-1 is conserved in all eukaryotes, and belongs to a family of lithium-sensitive phosphatases that includes inositol monophosphatase and inositol polyphosphate-1-phosphatase | Caenorhabditis elegans |
3.1.3.7 | malfunction | loss of BPNT-1 in Caenorhabditis elegans results in the selective dysfunction two neurons, the bilaterally symmetric pair of ASJ chemosensory neurons. As a result, BPNT-1 mutants are defective in behaviors dependent on the ASJ neurons, such as dauer exit and pathogen avoidance. Acute treatment with lithium also causes dysfunction of the ASJ neurons. This effect is reversible, and mediated specifically through inhibition of BPNT-1. The selective effect of lithium on the nervous system is due in part to the limited expression of the cytosolic sulfotransferase SSU-1 in the ASJ neuron pair. Lithium, through inhibition of BPNT-1 in the nervous system, can cause selective toxicity to specific neurons, resulting in corresponding effects on behavior of Caenorhabditis elegans, molecular mechanism by which loss of BPNT-1 leads to dysfunction of the ASJ neuron, bpnt-1 phenotypes, overview. The specific expression of the cytosolic sulfotransferase SSU-1 in the ASJ neuron pair may contribute to the selective neuronal dysfunction caused by loss of BPNT-1 | Caenorhabditis elegans |
3.1.3.7 | physiological function | bisphosphate 3'-nucleotidase (BPNT-1) is a lithium-sensitive phosphatase that catalyzes the breakdown of cytosolic 3'-phosphoadenosine-5'-phosphate (PAP), a byproduct of sulfation reactions utilizing the universal sulfate group donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). The daf-7 gene encodes a TGF-beta ligand that regulates diverse behaviors Caenorhabditis elegans and is transcriptionally activated in the ASJ chemosensory neuron pair upon exposure to the pathogenic bacteria Pseudomonas aeruginosa. BPNT-1 is required for the expression of daf-7 specifically in the ASJ neurons | Caenorhabditis elegans |