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Literature summary extracted from

  • Ghachi, M.E.; Howe, N.; Auger, R.; Lambion, A.; Guiseppi, A.; Delbrassine, F.; Manat, G.; Roure, S.; Peslier, S.; Sauvage, E.; Vogeley, L.; Rengifo-Gonzalez, J.C.; Charlier, P.; Mengin-Lecreulx, D.; Foglino, M.; Touze, T.; Caffrey, M.; Kerff, F.
    Crystal structure and biochemical characterization of the transmembrane PAP2 type phosphatidylglycerol phosphate phosphatase from Bacillus subtilis (2017), Cell. Mol. Life Sci., 74, 2319-2332 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.27 structure at 2.25 A resolution, with phosphate analogue tungstate in the active site. The structure reveals two lipid chains in the active site vicinity Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.27 G86F no significant change in activity Bacillus subtilis
3.1.3.27 K93A no significant change in activity with 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate), more than 50% increase in activity for undecaprenyldiphosphate and farnesyldiphosphate Bacillus subtilis
3.1.3.27 K93W no significant change in activity Bacillus subtilis
3.1.3.27 L82W no significant change in activity Bacillus subtilis
3.1.3.27 R89A slight decrease in activity Bacillus subtilis
3.1.3.27 S55A no significant change in activity Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.27 membrane
-
 Bacillus subtilis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.27 Bacillus subtilis O34349
-
-
3.1.3.27 Bacillus subtilis 168 O34349
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.27 12.5
-
 substrate undecaprenyldiphosphate, pH 7.4, 37°C Bacillus subtilis
3.1.3.27 19.8
-
 substrate isopentenyldiphosphate, pH 7.4, 37°C Bacillus subtilis
3.1.3.27 30.6
-
 substrate farnesyldiphosphate, pH 7.4, 37°C Bacillus subtilis
3.1.3.27 54.7
-
 substrate 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate), pH 7.4, 37°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.27 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O
-
 Bacillus subtilis 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate
-
 ?
3.1.3.27 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O
-
 Bacillus subtilis 168 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate
-
 ?
3.1.3.27 farnesyldiphosphate + H2O
-
 Bacillus subtilis farnesylphosphate + phosphate
-
 ?
3.1.3.27 farnesyldiphosphate + H2O
-
 Bacillus subtilis 168 farnesylphosphate + phosphate
-
 ?
3.1.3.27 isopentenyldiphosphate + H2O
-
 Bacillus subtilis isopentenylphosphate + phosphate
-
 ?
3.1.3.27 isopentenyldiphosphate + H2O
-
 Bacillus subtilis 168 isopentenylphosphate + phosphate
-
 ?
3.1.3.27 undecaprenyldiphosphate + H2O
-
 Bacillus subtilis undecaprenylphosphate + phosphate
-
 ?
3.1.3.27 undecaprenyldiphosphate + H2O
-
 Bacillus subtilis 168 undecaprenylphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.27 PgpB
-
 Bacillus subtilis
3.1.3.27 YodM
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
3.1.3.27 physiological function enzyme is the principal phosphatidylglycerol phosphate phosphatase of Bacillus subtilis. Gene expression complements both undecaprenylphosphate and phosphatidylglycerol metabolisms in Escherichia coli mutant strains Bacillus subtilis