Literature summary extracted from
Han, Y.Z.; Zhou, C.-C.; Xu, Y.-Y
; Yao, Y.-X.; Chi, Z.; Chi, Z.-M.; Liu, G.-L. High-efficient production of fructo-oligosaccharides from inulin by a two-stage bioprocess using an engineered Yarrowia lipolytica strain (2017), Carbohydr. Polym., 173, 592-599 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.7 |
gene inuA, recombinant overexpresssion in Yarrowia lipolytica strain Enop56 (engineered from strain Po1h), during fermentation with an inulin concentration of 600 g/l in a 10-l bioreactor, the inulinase activity, fructooligosaccharides (FOS) titer, yield, and productivity reach 551.6 U/ml, 546.6 g/l, 0.91 gFOS/g Inulin, and 15.18 g/l/h, respectively |
Aspergillus niger |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.7 |
Aspergillus niger |
O74641 |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.2.1.7 |
glycoprotein |
deglycosylation of the recombinant enzyme by endoglycosidase Endo H |
Aspergillus niger |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.7 |
recombinant enzyme from Yarrowia lipolytica strain ENop56 by dialysis and anion exchange chromatography, the enzyme is then deglycosylated by endoglycosidase Endo H, followed by gel filtration |
Aspergillus niger |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.7 |
1206.63 |
- |
purified recombinant enzyme, pH 4.5, 60°C |
Aspergillus niger |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.7 |
inulin + H2O |
- |
Aspergillus niger |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.7 |
? |
x * 56510, deglycosylated recombinant enzyme, SDS-PAGE |
Aspergillus niger |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.7 |
endo-inulinase |
- |
Aspergillus niger |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.7 |
60 |
- |
recombinant enzyme |
Aspergillus niger |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.2.1.7 |
35 |
70 |
maximal activity at 60°C, 70% activity at 35°C, 80% at 50°C and 70°C, profile overview |
Aspergillus niger |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.7 |
30 |
- |
purified recombinant enzyme, 120 min, 90% activity remaining |
Aspergillus niger |
3.2.1.7 |
60 |
- |
purified recombinant enzyme, 120 min, 60% activity remaining |
Aspergillus niger |
3.2.1.7 |
70 |
- |
purified recombinant enzyme, 120 min, 10% activity remaining |
Aspergillus niger |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.7 |
4.5 |
- |
recombinant enzyme |
Aspergillus niger |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.2.1.7 |
3 |
7 |
over 70% of maximal activity within this range, profile overview |
Aspergillus niger |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
3.2.1.7 |
2 |
8 |
purified recombinant enzyme, 120 min, over 50% activity remaining, completely stable at pH 4.5, profile overview |
Aspergillus niger |