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Literature summary extracted from

  • Rodriguez-Fuentes, G.; Marin-Lopez, V.; Hernandez-Marquez, E.
    Cholinesterases in Gambusia yucatana biochemical characterization and its relationship with sex and total length (2016), Bull. Environ. Contam. Toxicol., 97, 776-780 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide BW284c51, a specific inhibitor of AChE Gambusia yucatana
3.1.1.7 eserine
-
 Gambusia yucatana
3.1.1.8 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide BW284c51 Gambusia yucatana
3.1.1.8 eserine
-
 Gambusia yucatana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 additional information
-
 additional information Michaelis-Menten kinetics Gambusia yucatana
3.1.1.7 2.38
-
 acetylthiocholine enzyme from head, pH 7.4, temperature not specified in the publication Gambusia yucatana
3.1.1.7 5.47
-
 acetylthiocholine enzyme from muscle, pH 7.4, temperature not specified in the publication Gambusia yucatana
3.1.1.8 additional information
-
 additional information Michaelis-Menten kinetics Gambusia yucatana
3.1.1.8 0.55
-
 butyrylthiocholine enzyme from muscle, pH 7.4, temperature not specified in the publication Gambusia yucatana
3.1.1.8 1.24
-
 butyrylthiocholine enzyme from head, pH 7.4, temperature not specified in the publication Gambusia yucatana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.7 acetylcholine + H2O Gambusia yucatana
-
 choline + acetate
-
 ?
3.1.1.8 butyrylcholine + H2O Gambusia yucatana
-
 choline + butyrate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Gambusia yucatana
-
 from Yucatan Peninsula, Mexico
-
3.1.1.8 Gambusia yucatana
-
 from Yucatan Peninsula, Mexico
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 head
-
 Gambusia yucatana
-
3.1.1.7 muscle
-
 Gambusia yucatana
-
3.1.1.8 head
-
 Gambusia yucatana
-
3.1.1.8 additional information the ChE presented in head and muscle is able to hydrolyze ASChI and BSChI obtaining higher Vmax and Km with ASChI. This suggests that the ChE present in head and muscle have high affinity for BSChI (ASChI Km < BSChI Km) but is poorly hydrolyzed. With ASChI as substrate, a relatively high enzyme activity is obtained (ASChI Vmax > BSChI Vmax), but it has a smaller affinity for the enzyme with respect to BSChI Gambusia yucatana
-
3.1.1.8 muscle
-
 Gambusia yucatana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 0.12024
-
 pH 7.4, temperature not specified in the publication Gambusia yucatana
3.1.1.8 0.00595
-
 crude enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
 Gambusia yucatana choline + acetate
-
 ?
3.1.1.7 acetylthiocholine + H2O
-
 Gambusia yucatana thiocholine + acetate
-
 ?
3.1.1.8 butyrylcholine + H2O
-
 Gambusia yucatana choline + butyrate
-
 ?
3.1.1.8 butyrylthiocholine + H2O
-
 Gambusia yucatana thiocholine + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Gambusia yucatana
3.1.1.8 BChE
-
 Gambusia yucatana
3.1.1.8 butyrylcholinesterase
-
 Gambusia yucatana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.4
-
 assay at Gambusia yucatana
3.1.1.8 7.4
-
 assay at Gambusia yucatana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.00005
-
 head enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide
3.1.1.7 0.00006
-
 muscle enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide
3.1.1.7 0.00022
-
 head enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana eserine
3.1.1.7 0.00033
-
 muscle enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana eserine
3.1.1.8 0.00007
-
 head enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana eserine
3.1.1.8 0.00009
-
 muscle enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana eserine
3.1.1.8 0.00014
-
 muscle enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide
3.1.1.8 0.00037
-
 head enzyme, pH 7.4, temperature not specified in the publication Gambusia yucatana 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide

General Information

EC Number General Information Comment Organism
3.1.1.7 physiological function acetylcholinesterase (AChE) is a very important enzyme in the nervous system because it hydrolyzes acetylcholine in synaptic functions. Relationship between cholinesterase activity and total length or sex in wild Gambusia yucatana from different water bodies in the Yucatan Peninsula, Mexiko Gambusia yucatana