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Literature summary extracted from

  • Yakimova, B.; Tchorbanov, B.; Stoineva, I.
    Alpha-galactosidase and invertase from Penicillium chrysogenum sp.23 Purification, characteristics and hydrolysis of raffinose (2017), Bulg. Chem. Commun., 49, 101-106 .
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.22 raffinose + H2O Penicillium chrysogenum sp. 23
-
 D-fructose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Penicillium chrysogenum sp. 23
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 DEAE-cellulose column chromatography and Sephadex G-100 gel filtration Penicillium chrysogenum sp. 23

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 0.46
-
 lyophilized extract, at pH 5.5 and 50°C Penicillium chrysogenum sp. 23
3.2.1.22 30
-
 after 65.2fold purification, at pH 5.5 and 50°C Penicillium chrysogenum sp. 23

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Penicillium chrysogenum sp. 23 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 raffinose + H2O
-
 Penicillium chrysogenum sp. 23 D-fructose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-Gal
-
 Penicillium chrysogenum sp. 23

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 50
-
-
 Penicillium chrysogenum sp. 23

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 25 60 the enzyme is stable from 25 to 40°C for 24 h. At 45°C the activity falls to 80%, at 50°C to 72% and at 60°C the activity is completely lost Penicillium chrysogenum sp. 23

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4.5 5
-
 Penicillium chrysogenum sp. 23

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 4.5 6 the enzyme activity remains stable over a wide range of pH 4.5-6.0 for 24 h Penicillium chrysogenum sp. 23