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Literature summary extracted from

  • Yan, Q.; Fong, S.S.
    Cloning and characterization of a chitinase from Thermobifida fusca reveals Tfu_0580 as a thermostable and acidic endochitinase (2018), Biotechnol. Rep., 19, e00274 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Escherichia coli Thermobifida fusca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ 125% activity at 1 mM Thermobifida fusca
3.2.1.14 Mn2+ 119% activity at 1 mM Thermobifida fusca
3.2.1.14 additional information not influenced by K+, Na+, Mg2+, and Zn2+ Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Thermobifida fusca Q47SE9
-
-
3.2.1.14 Thermobifida fusca ATCC BAA-629 Q47SE9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 nickel-charged his-affinity gel chromatography Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O highest activity Thermobifida fusca ?
-
 ?
3.2.1.14 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O highest activity Thermobifida fusca ATCC BAA-629 ?
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Thermobifida fusca ATCC BAA-629 ?
-
 ?
3.2.1.14 colloidal chitin + H2O low activity Thermobifida fusca ?
-
 ?
3.2.1.14 colloidal chitin + H2O low activity Thermobifida fusca ATCC BAA-629 ?
-
 ?
3.2.1.14 additional information the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide Thermobifida fusca ?
-
 ?
3.2.1.14 additional information the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide Thermobifida fusca ATCC BAA-629 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 44900, SDS-PAGE Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 endochitinase
-
 Thermobifida fusca
3.2.1.14 Tfu_0580
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 30
-
-
 Thermobifida fusca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 20 65 the enzyme retains more than 80% activity between 20 and 55°C, while the chitinase activity drops to less than 40% when temperature is above 65°C Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
 Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4.8 7.5 the enzyme tends to be active under an acidic condition, which remain more than 60% highest activity between pH 4.8 and 7.5. When pH is at 8.0, the enzyme activity drops to 40% of its highest activity Thermobifida fusca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Thermobifida fusca calculated from amino acid sequence
-
 5.8