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Literature summary extracted from

  • Inmaculada, N.; Francisco, G.
    Characterization of cross-linked immobilized arylesterase from Gluconobacter oxydans 621H with activity toward cephalosporin C and 7-aminocephalosporanic acid (2016), Biotechnol. Prog., 32, 36-42 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 SDS complete inhibition at 5 and 10% (w/v) Gluconobacter oxydans
3.1.1.2 Triton X-100 61% residual activity at 10% (v/v) Gluconobacter oxydans
3.1.1.2 Tween 20 51% residual activity at 10% (v/v) Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.014
-
 2-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 0.039
-
 1-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 0.069
-
 phenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 0.072
-
 4-nitrophenyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 0.094
-
 4-nitrophenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 0.1
-
 2-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 0.369
-
 1-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 7.1
-
 S-methyl thiobutanoate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 74.6
-
 7-aminocephalosporanic acid immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 98.7
-
 7-aminocephalosporanic acid free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 119
-
 cephalosporin C free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 174.7
-
 cephalosporin C immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.2 additional information the enzyme does not require a metal cofactor Gluconobacter oxydans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.2 Acetone in the presence of 40% (v/v) acetone, the cross-linked enzyme aggregate maintains 80% activity while the native enzyme totally loses its activity Gluconobacter oxydans
3.1.1.2 acetonitrile in the presence of 40% (v/v) acetonitrile, the cross-linked enzyme aggregate maintains 33% activity Gluconobacter oxydans
3.1.1.2 DMSO in the presence of 40% (v/v) DMSO, the cross-linked enzyme aggregate maintains 73% activity Gluconobacter oxydans
3.1.1.2 Ethanol with 40% (v/v) ethanol, the cross-linked enzyme aggregate retains 100% activity, whereas the free enzyme loses its activity Gluconobacter oxydans
3.1.1.2 additional information the addition of organic solvents, such as methanol, ethanol, acetonitrile, acetone, DMSO, and propanol, had little effect on activity when used at 5% and 10% (v/v) Gluconobacter oxydans
3.1.1.2 propanol in the presence of 40% (v/v) propanol, the cross-linked enzyme aggregate maintains 11% activity Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Gluconobacter oxydans
-
-
-
3.1.1.2 Gluconobacter oxydans 621H
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 1-naphthyl acetate + H2O
-
 Gluconobacter oxydans 1-naphthol + acetate
-
 ?
3.1.1.2 1-naphthyl acetate + H2O
-
 Gluconobacter oxydans 621H 1-naphthol + acetate
-
 ?
3.1.1.2 1-naphthyl butyrate + H2O
-
 Gluconobacter oxydans 1-naphthol + butyrate
-
 ?
3.1.1.2 2-naphthyl acetate + H2O
-
 Gluconobacter oxydans 2-naphthol + acetate
-
 ?
3.1.1.2 2-naphthyl acetate + H2O
-
 Gluconobacter oxydans 621H 2-naphthol + acetate
-
 ?
3.1.1.2 2-naphthyl butyrate + H2O
-
 Gluconobacter oxydans 2-naphthol + butyrate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Gluconobacter oxydans 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Gluconobacter oxydans 621H 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl butyrate + H2O best substrate Gluconobacter oxydans 4-nitrophenol + butyrate
-
 ?
3.1.1.2 4-nitrophenyl butyrate + H2O best substrate Gluconobacter oxydans 621H 4-nitrophenol + butyrate
-
 ?
3.1.1.2 4-nitrophenyl caprylate + H2O
-
 Gluconobacter oxydans 4-nitrophenol + caprylate
-
 ?
3.1.1.2 4-nitrophenyl laurate + H2O
-
 Gluconobacter oxydans 4-nitrophenol + laurate
-
 ?
3.1.1.2 7-aminocephalosporanic acid + H2O
-
 Gluconobacter oxydans ?
-
 ?
3.1.1.2 cephalosporin C + H2O
-
 Gluconobacter oxydans ?
-
 ?
3.1.1.2 phenyl acetate + H2O
-
 Gluconobacter oxydans phenol + acetate
-
 ?
3.1.1.2 phenyl acetate + H2O
-
 Gluconobacter oxydans 621H phenol + acetate
-
 ?
3.1.1.2 S-methyl thiobutanoate + H2O
-
 Gluconobacter oxydans methanol + thiobutanoate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 40
-
 native enzyme Gluconobacter oxydans
3.1.1.2 50
-
 cross-linked enzyme aggregate Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.2 50 60 after 5 min of incubation at 60°C, 20 min at 55°C and 40 min at 50°C, the immobilized cross-linked enzyme aggregate loses all of its initial activity. However, at 40°C, it is still slightly active after 7 h incubation Gluconobacter oxydans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.2 4.7
-
 1-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 9.3
-
 S-methyl thiobutanoate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 9.8
-
 1-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 15.2
-
 2-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 17.6
-
 4-nitrophenyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 20.81
-
 cephalosporin C immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 22.7
-
 2-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 25.7
-
 4-nitrophenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 42.7
-
 cephalosporin C free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 61.3
-
 7-aminocephalosporanic acid free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 69.5
-
 7-aminocephalosporanic acid immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 102
-
 phenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 7
-
 native enzyme Gluconobacter oxydans
3.1.1.2 8
-
 cross-linked enzyme aggregate Gluconobacter oxydans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.2 0.1
-
 cephalosporin C immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 0.9
-
 7-aminocephalosporanic acid immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 1
-
 S-methyl thiobutanoate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 20
-
 1-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 110
-
 1-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 220
-
 2-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 240
-
 4-nitrophenyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 270
-
 4-nitrophenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 1100
-
 2-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 1480
-
 phenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
3.1.1.2 2400
-
 7-aminocephalosporanic acid free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
3.1.1.2 2700
-
 cephalosporin C free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans