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Literature summary extracted from

  • Liu, Y.J.; Liu, S.; Dong, S.; Li, R.; Feng, Y.; Cui, Q.
    Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum (2018), Biotechnol. Biofuels, 11, 6-6 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 6.84
-
 Avicel at pH 5.7 and 55°C Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Acetivibrio thermocellus
-
-
-
3.2.1.176 Acetivibrio thermocellus DSM1313
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 Ni2+ Sepharose column chromatography and Superdex S200 gel filtration Acetivibrio thermocellus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.176
-
 Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 avicel + H2O 100% activity Acetivibrio thermocellus ?
-
 ?
3.2.1.176 avicel + H2O 100% activity Acetivibrio thermocellus DSM1313 ?
-
 ?
3.2.1.176 carboxymethyl cellulose + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.176 carboxymethyl cellulose + H2O
-
 Acetivibrio thermocellus DSM1313 ?
-
 ?
3.2.1.176 cellopentaose + H2O about 75% activity compared to Avicel Acetivibrio thermocellus ?
-
 ?
3.2.1.176 cellopentaose + H2O about 75% activity compared to Avicel Acetivibrio thermocellus DSM1313 ?
-
 ?
3.2.1.176 cellotetraose + H2O about 20% activity compared to Avicel Acetivibrio thermocellus ?
-
 ?
3.2.1.176 cellotetraose + H2O about 20% activity compared to Avicel Acetivibrio thermocellus DSM1313 ?
-
 ?
3.2.1.176 crystalline cellulose + H2O preferred substrate Acetivibrio thermocellus ?
-
 ?
3.2.1.176 additional information no activity with pectin, xylan, cellobiose and cellotriose Acetivibrio thermocellus ?
-
 ?
3.2.1.176 additional information no activity with pectin, xylan, cellobiose and cellotriose Acetivibrio thermocellus DSM1313 ?
-
 ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O about 30% activity compared to Avicel Acetivibrio thermocellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.176 ? x * 66000, SDS-PAGE Acetivibrio thermocellus
3.2.1.176 ? x * 74268, His12-tagged GH domain, calculated from amino acid sequence Acetivibrio thermocellus
3.2.1.176 ? x * 74272, catalytic domain, HPLC-Q-TOF mass spectrometry Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel48S
-
 Acetivibrio thermocellus
3.2.1.176 exoglucanase
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 70
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.176 55 80 more than 70% activity between 55 and 80°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 5.7
-
-
 Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.176 4.5 7 more than 85% activity between pH 4.5 and 6.5, about 75% activity at pH 7.0 Acetivibrio thermocellus

General Information

EC Number General Information Comment Organism
3.2.1.176 physiological function major exoglucanase of the cellulosome Acetivibrio thermocellus