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Literature summary extracted from

  • Corbin, J.M.; Kailemia, M.J.; Cadieux, C.L.; Alkanaimsh, S.; Karuppanan, K.; Rodriguez, R.L.; Lebrilla, C.B.; Cerasoli, D.M.; McDonald, K.A.; Nandi, S.
    Purification, characterization, and N-glycosylation of recombinant butyrylcholinesterase from transgenic rice cell suspension cultures (2018), Biotechnol. Bioeng., 115, 1301-1310 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.8 medicine the efficient downstream processing scheme and functionality of regulated transgenic enzyme rrBChE confirm its promise as a cost-effective alternative to human BChE for prophylactic and therapeutic use Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 additional information recombinant regulated BChE (rrBChE) differs from the wild-type in that it contains plant-type complex N-glycans, including an alpha-1,3 linked core fucose, and a beta-1,2 xylose, and lacking a terminal sialic acid. Despite this difference, rrBChE is demonstrated to be an effective stoichiometric bioscavenger for five different organophosphorous nerve agents in vitro Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 cyclosarin strongest inhibitor Oryza sativa
3.1.1.8 huprine X
-
 Oryza sativa
3.1.1.8 O-ethyl-S-2-diisopropylaminoethylmethylphosphonothiolate VX Oryza sativa
3.1.1.8 sarin
-
 Oryza sativa
3.1.1.8 soman
-
 Oryza sativa
3.1.1.8 Tabun
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.112
-
 butyrylthiocholine pH 7.4, 25°C, recombinant enzyme Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Oryza sativa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.8 glycoprotein site-specific and overall N-glycan structures on recombinant rBChE, glycosylation sites are N47, N241, N256, N341, N481, N455, N485, N481, and N486 Oryza sativa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8 active recombinant enzyme rrBChE from rice cells to 95% purity by tangential flow ultrafiltration, anion exchange chromatography, and Hupresin affinity chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 cell suspension culture
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 butyrylthiocholine + H2O
-
 Oryza sativa thiocholine + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 BChE
-
 Oryza sativa
3.1.1.8 butyrylcholinesterase
-
 Oryza sativa
3.1.1.8 rBChE
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.8 25
-
 assay at Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.8 10 60 purified recombinant enzyme, pH 7.4, 30 min, completely stable at up to 20°C, loss of about 20% activity at 40°C and of 75% at 50°C, inactivation at 60°C Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 2066.7
-
 butyrylthiocholine pH 7.4, 25°C, recombinant enzyme Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7.4
-
 assay at Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.8 5 8 the enzyme shows very low (but non-zero) activity at pH 5.0, with a gradual rise in activity to pH 8.0 Oryza sativa

General Information

EC Number General Information Comment Organism
3.1.1.8 additional information structure and activity comparisons of Oryzsa sativa and Homo sapiens enzymes, overview Oryza sativa