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Literature summary extracted from
- Comprehensive kinetic and substrate specificity analysis of an arylsulfatase from Helix pomatia using mass spectrometry (2019), Bioorg. Med. Chem., 27, 955-962 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.6.1 | analysis | development of a time-based mass spectrometric assay to analyze substrate conversion and substrate specificity | Helix pomatia |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.1 | 0.112 | - |
methylurolithin A sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.131 | - |
resorcinol sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia |  |
| 3.1.6.1 | 0.161 | - |
ferulic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.175 | - |
4-Methylumbelliferyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.216 | - |
Indoxyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.262 | - |
p-coumaric acid sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.341 | - |
vanillic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.351 | - |
Estrone 3-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 1.02 | - |
4-ethylphenyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 1.4 | - |
p-cresyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 1.486 | - |
N-acetyltyramine sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.1 | Helix pomatia | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.1 | 4-ethylphenyl sulfate + H2O | - |
Helix pomatia | 4-ethylphenol + sulfate | - |
? | |
| 3.1.6.1 | 4-methylumbelliferyl sulfate + H2O | - |
Helix pomatia | 4-methylumbelliferone + sulfate | - |
? | |
| 3.1.6.1 | estrone 3-sulfate + H2O | - |
Helix pomatia | estrone + sulfate | - |
? | |
| 3.1.6.1 | ferulic acid 4-sulfate + H2O | - |
Helix pomatia | ferulic acid + sulfate | - |
? | |
| 3.1.6.1 | indoxyl sulfate + H2O | - |
Helix pomatia | indoxol + sulfate | - |
? | |
| 3.1.6.1 | methylurolithin A sulfate + H2O | - |
Helix pomatia | methylurolithin A + sulfate | - |
? | |
| 3.1.6.1 | N-acetyltyramine sulfate + H2O | - |
Helix pomatia | N-acetyltyramine + sulfate | - |
? | |
| 3.1.6.1 | p-coumaric acid sulfate + H2O | - |
Helix pomatia | p-coumaric acid + sulfate | - |
? | |
| 3.1.6.1 | p-cresyl sulfate + H2O | - |
Helix pomatia | p-cresol + sulfate | - |
? | |
| 3.1.6.1 | resorcinol sulfate + H2O | - |
Helix pomatia | resorcinol + sulfate | - |
? | |
| 3.1.6.1 | vanillic acid 4-sulfate + H2O | - |
Helix pomatia | vanillic acid + sulfate | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.1 | 92 | - |
resorcinol sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 119 | - |
vanillic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 138 | - |
ferulic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 228 | - |
Indoxyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 235 | - |
p-coumaric acid sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 871 | - |
p-cresyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 1800 | - |
N-acetyltyramine sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 7150 | - |
4-ethylphenyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 28600 | - |
4-Methylumbelliferyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 40900 | - |
Estrone 3-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 88500 | - |
methylurolithin A sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.1 | 0.35 | - |
vanillic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.62 | - |
p-cresyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.69 | - |
resorcinol sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.86 | - |
ferulic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 0.9 | - |
p-coumaric acid sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 1.06 | - |
Indoxyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 1.2 | - |
N-acetyltyramine sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 7 | - |
4-ethylphenyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 117 | - |
Estrone 3-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 164 | - |
4-Methylumbelliferyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 3.1.6.1 | 787 | - |
methylurolithin A sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia |
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