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Literature summary extracted from

  • Belhaj, I.; Amara, S.; Parsiegla, G.; Sutto-Ortiz, P.; Sahaka, M.; Belghith, H.; Rousset, A.; Lafont, D.; Carriere, F.
    Galactolipase activity of Talaromyces thermophilus lipase on galactolipid micelles, monomolecular films and UV-absorbing surface-coated substrate (2018), Biochim. Biophys. Acta, 1863, 1006-1015 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Thermomyces dupontii F6LQK7
-
-
3.1.1.3 Thermomyces dupontii Stolk F6LQK7
-
-
3.1.1.26 Thermomyces dupontii F6LQK7
-
-
3.1.1.26 Thermomyces dupontii Stolk F6LQK7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme from strain Stolk to homogeneity Thermomyces dupontii
3.1.1.26 native enzyme from strain Stolk to homogeneity Thermomyces dupontii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 4250
-
 substrate phosphatidylcholine, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
3.1.1.3 7300
-
 substrate tributyrin, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
3.1.1.3 9808
-
 substrate olive oil, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
3.1.1.3 24110
-
 substrate trioctanoin, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
3.1.1.26 4250
-
 substrate phosphatidylcholine, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
3.1.1.26 9800
-
 substrate 3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-octanoyl-sn-glycerol, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
3.1.1.26 40500
-
 substrate 1,2-di-O-alpha-eleostearoyl-3-O-beta-D-galactopyranosyl-sn-glycerol, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information Talaromyces thermophilus lipase (TTL) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates (EC 3.1.1.26) presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii ?
-
 ?
3.1.1.3 additional information Talaromyces thermophilus lipase (TTL) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates (EC 3.1.1.26) presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii Stolk ?
-
 ?
3.1.1.3 olive oil + H2O
-
 Thermomyces dupontii ?
-
 ?
3.1.1.3 olive oil + H2O
-
 Thermomyces dupontii Stolk ?
-
 ?
3.1.1.3 phosphatidylcholine + H2O from hen egg Thermomyces dupontii ?
-
 ?
3.1.1.3 phosphatidylcholine + H2O from hen egg Thermomyces dupontii Stolk ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Thermomyces dupontii dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O
-
 Thermomyces dupontii Stolk dibutyrin + butyrate
-
 ?
3.1.1.3 trioctanoin + H2O
-
 Thermomyces dupontii dioctanoin + octanoate
-
 ?
3.1.1.3 trioctanoin + H2O
-
 Thermomyces dupontii Stolk dioctanoin + octanoate
-
 ?
3.1.1.26 1,2-di-O-alpha-eleostearoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O best substrate Thermomyces dupontii ? + alpha-eleostearic acid
-
 ?
3.1.1.26 3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-octanoyl-sn-glycerol + H2O
-
 Thermomyces dupontii ?
-
 ?
3.1.1.26 additional information Talaromyces thermophilus lipase (TTL, EC 3.1.1.3) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii ?
-
 ?
3.1.1.26 phosphatidylcholine + H2O from hen egg Thermomyces dupontii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 Talaromyces thermophilus lipase
-
 Thermomyces dupontii
3.1.1.3 TTL
-
 Thermomyces dupontii
3.1.1.26 Talaromyces thermophilus lipase
-
 Thermomyces dupontii
3.1.1.26 TTL
-
 Thermomyces dupontii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Thermomyces dupontii
3.1.1.26 37
-
 assay at Thermomyces dupontii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Thermomyces dupontii
3.1.1.26 8
-
-
 Thermomyces dupontii

General Information

EC Number General Information Comment Organism
3.1.1.3 additional information three-dimensional structure molecular modelling and dynamic light scattering measurements, sequence comparisons, overview Thermomyces dupontii
3.1.1.26 additional information three-dimensional structure molecular modelling and dynamic light scattering measurements, sequence comparisons, overview Thermomyces dupontii