Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Taylor, Z.W.; Raushel, F.M.
    Cytidine diphosphoramidate kinase an enzyme required for the biosynthesis of the O-methyl phosphoramidate modification in the capsular polysaccharides of Campylobacter jejuni (2018), Biochemistry, 57, 2238-2244 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.224 expression in Escherichia coli Campylobacter jejuni subsp. jejuni serotype O:2

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.224 S85A about 5% of wild-type catalytic efficiency Campylobacter jejuni subsp. jejuni serotype O:2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.224 0.11
-
 cytidine 5'-diphosphoramidate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 0.39
-
 2'-deoxycytidine 5'-diphosphoramidate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 0.7
-
 ATP mutant S85A, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 0.8
-
 CDP-methyl phosphonate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 1.5
-
 ATP wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 4.4
-
 cytidine 5'-diphosphoramidate mutant S85A, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.13 ATP + L-glutamine + H2O Campylobacter jejuni
-
 AMP + phosphate + N5-phospho-L-glutamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.224 Campylobacter jejuni subsp. jejuni serotype O:2 Q0P8J9
-
-
2.7.1.224 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0P8J9
-
-
2.7.3.13 Campylobacter jejuni
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.224 ATP + 2'-deoxycytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ADP + 2'-deoxycytidine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + 2'-deoxycytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ADP + 2'-deoxycytidine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + adenosine 5'-diphosphoramidate poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ADP + adenosine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + adenosine 5'-diphosphoramidate poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ADP + adenosine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + CDP-methyl phosphonate
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ADP + ?
-
 ?
2.7.1.224 ATP + CDP-methyl phosphonate
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ADP + ?
-
 ?
2.7.1.224 ATP + cytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ADP + cytidine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + cytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ADP + cytidine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + uridine 5'-diphosphoramidate poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ADP + uridine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.1.224 ATP + uridine 5'-diphosphoramidate poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ADP + uridine 3'-phospho-5'-diphosphoramidate + H+
-
 ?
2.7.3.13 ATP + L-glutamine + H2O
-
 Campylobacter jejuni AMP + phosphate + N5-phospho-L-glutamine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.224 Cj1415c
-
 Campylobacter jejuni subsp. jejuni serotype O:2
2.7.3.13 Cj1418
-
 Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.224 1.4
-
 CDP-methyl phosphonate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 1.6
-
 ATP mutant S85A, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 1.6
-
 cytidine 5'-diphosphoramidate mutant S85A, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 2.2
-
 ATP wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 2.2
-
 cytidine 5'-diphosphoramidate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 2.2
-
 2'-deoxycytidine 5'-diphosphoramidate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

General Information

EC Number General Information Comment Organism
2.7.3.13 metabolism the enzyme is required for the biosynthesis of the phosphoramidate group of O-methyl phosphoramidate Campylobacter jejuni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.224 0.36
-
 cytidine 5'-diphosphoramidate mutant S85A, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 1.5
-
 ATP wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 1.7
-
 CDP-methyl phosphonate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 2.3
-
 ATP mutant S85A, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 5.6
-
 2'-deoxycytidine 5'-diphosphoramidate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.1.224 20
-
 cytidine 5'-diphosphoramidate wild-type, pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2