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Literature summary extracted from

  • Bigley, A.N.; Narindoshvili, T.; Xiang, D.F.; Raushel, F.M.
    Multiple reaction products from the hydrolysis of chiral and prochiral organophosphate substrates by the phosphotriesterase from Sphingobium sp. TCM1 (2018), Biochemistry, 57, 1842-1846 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.8.1 0.006
-
 cyclohexyl methyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.036
-
 methyl 4-nitrophenyl propan-2-yl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.053
-
 cyclohexyl methyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.054
-
 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.059
-
 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.061
-
 methyl 4-nitrophenyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.08
-
 methyl bis(4-nitrophenyl) phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.083
-
 diethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.12
-
 4-acetylphenyl cyclohexyl methyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.25
-
 methyl 4-nitrophenyl phenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.31
-
 4-acetylphenyl cyclohexyl methyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.76
-
 dimethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 1.1
-
 cyclohexyl methyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 2.3
-
 methyl 4-nitrophenyl propan-2-yl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.8.1 additional information enzyme Sb-PTE uses a binuclear metal center Sphingobium sp. TCM1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.8.1 additional information Sphingobium sp. TCM1 phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) possesses a rather broad substrate profile and is able to hydrolyze insecticides, plasticizers, and flame retardants that are not typically substrates for other enzymes of this class ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Sphingobium sp. TCM1 A0A077JBW9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.1 4-acetylphenyl cyclohexyl methyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (R)-phosphate + 4-acetylphenol
-
 ?
3.1.8.1 4-acetylphenyl cyclohexyl methyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (S)-phosphate + 4-acetylphenol
-
 ?
3.1.8.1 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl ethyl (R)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl ethyl (S)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 cyclohexyl methyl 4-nitrophenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (R)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 cyclohexyl methyl 4-nitrophenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (S)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 cyclohexyl methyl phenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (R)-phosphate + phenol
-
 ?
3.1.8.1 cyclohexyl methyl phenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (S)-phosphate + phenol
-
 ?
3.1.8.1 diethyl 4-nitrophenyl phosphate + H2O
-
 Sphingobium sp. TCM1 diethyl phosphate + 4-nitrophenol
-
 ?
3.1.8.1 dimethyl 4-nitrophenyl phosphate + H2O
-
 Sphingobium sp. TCM1 dimethyl phosphate + 4-nitrophenol
-
 ?
3.1.8.1 methyl 4-nitrophenyl phenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl phenyl (R)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 methyl 4-nitrophenyl phenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl phenyl (S)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 methyl 4-nitrophenyl propan-2-yl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl propan-2-yl (R)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 methyl 4-nitrophenyl propan-2-yl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl propan-2-yl (S)-phosphate + 4-nitrophenol
-
 ?
3.1.8.1 methyl bis(4-nitrophenyl) phosphate + H2O
-
 Sphingobium sp. TCM1 methyl phosphate + 2 4-nitrophenol
-
 ?
3.1.8.1 additional information phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) possesses a rather broad substrate profile and is able to hydrolyze insecticides, plasticizers, and flame retardants that are not typically substrates for other enzymes of this class Sphingobium sp. TCM1 ?
-
 ?
3.1.8.1 additional information multiple reaction products from the hydrolysis of chiral and prochiral organophosphate substrates, substrate specificity and differential hydrolysis of the prochiral substituents, analysis of the enantioselectivity, overview Sphingobium sp. TCM1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 A7Q26_23485
-
 Sphingobium sp. TCM1
3.1.8.1 HAD
-
 Sphingobium sp. TCM1
3.1.8.1 haloalkylphosphorus hydrolase UniProt Sphingobium sp. TCM1
3.1.8.1 Sb-PTE
-
 Sphingobium sp. TCM1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.8.1 30
-
 assay at Sphingobium sp. TCM1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.8.1 0.36
-
 4-acetylphenyl cyclohexyl methyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.38
-
 4-acetylphenyl cyclohexyl methyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.38
-
 cyclohexyl methyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 0.6
-
 methyl 4-nitrophenyl phenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 1.11
-
 cyclohexyl methyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 2.05
-
 methyl 4-nitrophenyl propan-2-yl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 2.5
-
 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 2.8
-
 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 5
-
 methyl 4-nitrophenyl propan-2-yl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 6
-
 cyclohexyl methyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 6.3
-
 methyl 4-nitrophenyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 6.5
-
 diethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 9
-
 methyl bis(4-nitrophenyl) phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 9.1
-
 dimethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.8.1 8.5
-
 assay at Sphingobium sp. TCM1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.8.1 1.16
-
 4-acetylphenyl cyclohexyl methyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 2.17
-
 methyl 4-nitrophenyl propan-2-yl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 2.4
-
 methyl 4-nitrophenyl phenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 3.17
-
 4-acetylphenyl cyclohexyl methyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 5.45
-
 cyclohexyl methyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 11.97
-
 dimethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 20.94
-
 cyclohexyl methyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 42.37
-
 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 51.85
-
 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 56.94
-
 methyl 4-nitrophenyl propan-2-yl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 63.33
-
 cyclohexyl methyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 78.31
-
 diethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 103.28
-
 methyl 4-nitrophenyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.1.8.1 112.5
-
 methyl bis(4-nitrophenyl) phosphate pH 8.5, 30°C Sphingobium sp. TCM1