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Literature summary extracted from

  • Alteen, M.G.; Oehler, V.; Nemcovicova, I.; Wilson, I.B.; Vocadlo, D.J.; Gloster, T.M.
    Mechanism of human nucleocytoplasmic hexosaminidase D (2016), Biochemistry, 55, 2735-2747 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.52 D148A the mutant shows 868fold decreased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
3.2.1.52 D148N the mutant shows 4190fold decreased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
3.2.1.52 E149A the mutant shows 1.6fold decreased catalytic efficiency with 4-nitrophenyl N-acetyl-beta-D-galactosaminide and 0.85fold increased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
3.2.1.52 E149Q the mutant shows 2.2fold decreased catalytic efficiency with 4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
3.2.1.52 H92A the mutant shows 69fold decreased catalytic efficiency with 4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Gal-NAG-thiazoline
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.0525
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.0721
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.102
-
 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.103
-
 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.172
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.175
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149Q, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.196
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme H92A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.213
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.533
-
 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.566
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.607
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.852
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 1.73
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148N, at pH 7.4 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 nucleoplasm
-
 Homo sapiens 5654
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 HisTrap nickel column chromatography and S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Homo sapiens 3-fluoro-4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Homo sapiens 3-fluoro-4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + 2-deoxy-2-difluoroacetamido-beta-D-galactopyranose
-
 ?
3.2.1.52 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + 2-deoxy-2-fluoroacetamido-beta-D-galactopyranose
-
 ?
3.2.1.52 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranose
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 HexD
-
 Homo sapiens
3.2.1.52 HEXDC
-
 Homo sapiens
3.2.1.52 hexosaminidase D
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 0.0048
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148N, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.0077
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.042
-
 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.0515
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme H92A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.27
-
 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.51
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.6016
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 1.32
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 1.4883
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149Q, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 1.54
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 2.03
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 2.6
-
 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 2.9333
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6.5 7
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.52 0.00042
-
 Gal-NAG-thiazoline wild type enzyme, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.52 0.0028
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148N, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.0136
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.26
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme H92A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.41
-
 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 0.835
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 1.817
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 2.65
-
 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 4.98
-
 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 8.47
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149Q, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 11.52
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 11.783
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 13.75
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
3.2.1.52 18.167
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens