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Literature summary extracted from

  • Spencer, C.; Brown, H.A.
    Biochemical characterization of a Pseudomonas aeruginosa phospholipase D (2015), Biochemistry, 54, 1208-1218 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4
-
 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 Ca2+ inhibitory at 5 mM and 10 mM, substrate dioleoylphosphatidylethanolamine, stimulation at 2.5 mM, substrate dioleoylphosphatidylcholine Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ inhibitory at 5 mM and 10 mM, substrate dioleoylphosphatidylethanolamine. Presence of Ca2+ is required for substrate dioleoylphosphatidylcholine Pseudomonas aeruginosa
3.1.4.4 Mg2+ 10 mM, 1.6fold increase in activity, substrate dioleoylphosphatidylethanolamine. Presence of Mg2+ is required for substrate dioleoylphosphatidylcholine Pseudomonas aeruginosa
3.1.4.4 additional information stimulation by divalent cation depends on the substrate Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Pseudomonas aeruginosa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 1345
-
 substrate oleoylphosphatidylethanolamine, pH 7.5, 37°C Pseudomonas aeruginosa
3.1.4.4 2871
-
 substrate dioleoylphosphatidylethanolamine, pH 7.5, 37°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 1-oleoyl-2-stearoylphosphatidylethanolamine + H2O
-
 Pseudomonas aeruginosa ethanolamine + 1-oleoyl-2-stearoyl-sn-glycero-3-phosphatidic acid
-
 ?
3.1.4.4 dioleoylphosphatidylcholine + H2O
-
 Pseudomonas aeruginosa choline + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid
-
 ?
3.1.4.4 dioleoylphosphatidylethanolamine + H2O
-
 Pseudomonas aeruginosa ethanolamine + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid
-
 ?
3.1.4.4 additional information PldA is capable of using methanol, ethanol, and butanol to produce transphosphatidylation products Pseudomonas aeruginosa ?
-
 ?
3.1.4.4 oleoyllysophosphatidylethanolamine + H2O
-
 Pseudomonas aeruginosa ethanolamine + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 PldA
-
 Pseudomonas aeruginosa