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Literature summary extracted from

  • Phansopa, C.; Kozak, R.P.; Liew, L.P.; Frey, A.M.; Farmilo, T.; Parker, J.L.; Kelly, D.J.; Emery, R.J.; Thomson, R.I.; Royle, L.; Gardner, R.A.; Spencer, D.I.; Stafford, G.P.
    Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase (2015), Biochem. J., 472, 157-167 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.53 expressed in Escherichia coli BL21(DE3) Origami B cells Tannerella forsythia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.53 0.0512
-
 4-nitrophenyl acetate at pH 7.4 and 25°C Tannerella forsythia

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.53 Tannerella forsythia G8UJ84
-
-
3.1.1.53 Tannerella forsythia 92A.2 G8UJ84
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.53 GSTrap column chromatography, and Superdex 200 gel filtration Tannerella forsythia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.53 4-nitrophenyl acetate + H2O
-
 Tannerella forsythia 4-nitrophenol + acetate
-
 ?
3.1.1.53 4-nitrophenyl acetate + H2O
-
 Tannerella forsythia 92A.2 4-nitrophenol + acetate
-
 ?
3.1.1.53 additional information the enzyme show no activity with N-acetyl-O-5,4-diacetylneuraminate Tannerella forsythia ?
-
 ?
3.1.1.53 additional information the enzyme show no activity with N-acetyl-O-5,4-diacetylneuraminate Tannerella forsythia 92A.2 ?
-
 ?
3.1.1.53 N-acetyl-O-5,9-diacetylneuraminate + H2O
-
 Tannerella forsythia N-acetylneuraminate + acetate
-
 ?
3.1.1.53 N-acetyl-O-5,9-diacetylneuraminate + H2O
-
 Tannerella forsythia 92A.2 N-acetylneuraminate + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.53 ? x * 76300, mature protein, calculated from amino acid sequence Tannerella forsythia

Synonyms

EC Number Synonyms Comment Organism
3.1.1.53 NANS
-
 Tannerella forsythia
3.1.1.53 sialate-O-acetylesterase
-
 Tannerella forsythia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.53 111
-
 4-nitrophenyl acetate at pH 7.4 and 25°C Tannerella forsythia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.53 2200
-
 4-nitrophenyl acetate at pH 7.4 and 25°C Tannerella forsythia