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Literature summary extracted from

  • Ku, B.; Keum, C.W.; Lee, H.S.; Yun, H.Y.; Shin, H.C.; Kim, B.Y.; Kim, S.J.
    Crystal structure of SP-PTP, a low molecular weight protein tyrosine phosphatase from Streptococcus pyogenes (2016), Biochem. Biophys. Res. Commun., 478, 1217-1222 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 gene SPy_0039, recombinant expression of His-tagged enzyme Sp-PTP in Escherichia coli strain BL21(DE3) Streptococcus pyogenes serotype M1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.48 purified recombinant His-tagged enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM 2-mercaptoethanol, and 2 mM dithiothreitol, with 0.001 ml of precipitant solution containing 150 mM potassium thiocyanate, 100 mM Bis-Tris, pH 6.25, 8% PEG 3350, and 2% D-sorbitol, 18°C, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement method using the structure of the YfkJ from Bacillus subtilis as a search model Streptococcus pyogenes serotype M1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.48 [a protein]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M1
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Streptococcus pyogenes serotype M1 Q9A1Y0 a Group A Streptococcus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 recombinant His-tagged enzyme Sp-PTP from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptococcus pyogenes serotype M1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Streptococcus pyogenes serotype M1 4-nitrophenol + phosphate
-
 ?
3.1.3.48 additional information enzyme SP-PTP shares high similarity with typical low molecular weight protein tyrosine phosphatases (LMWPTPs), which are specific for phosphotyrosine, but not with dual-specificity phosphatases, in overall folding and active site composition. In the dephosphorylation activity test, SP-PTP consistently acts on phosphotyrosine substrates, but not or only minimally on phosphoserine/phosphothreonine substrates. PTP as a canonical tyrosine-specific LMWPTP Streptococcus pyogenes serotype M1 ?
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M1 [a protein]-tyrosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.48 More the SP-PTP molecule is composed of four parallel beta-strands [beta1(Lys3-Val7), beta2(Met32-Ala37), beta3(Tyr81-Gly84), and beta4(Ile105-Leu107)] sandwiched on both sides by five alpha-helices [a1(Cys13-Ile25), alpha2(Ser49-Tyr58), alpha3(Ile73-Thr78), alpha4(Ser87-Ser97), and alpha5(Phe122-Leu140)], which are arranged to form the Rossmann fold, which is typical of LMWPTPs Streptococcus pyogenes serotype M1

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 LMWPTP
-
 Streptococcus pyogenes serotype M1
3.1.3.48 low molecular weight protein tyrosine phosphatase
-
 Streptococcus pyogenes serotype M1
3.1.3.48 SP-PTP
-
 Streptococcus pyogenes serotype M1
3.1.3.48 SPy_0039
-
 Streptococcus pyogenes serotype M1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 6.5
-
 assay at Streptococcus pyogenes serotype M1

General Information

EC Number General Information Comment Organism
3.1.3.48 additional information enzyme structure analysis and comparisons, overview Streptococcus pyogenes serotype M1