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Literature summary extracted from
- Characterization of tetrachlorohydroquinone reductive dehalogenase from Sphingomonas sp. UG30 (2002), Biochem. Biophys. Res. Commun., 299, 634-640 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.4.5 | dithiothreitol | increasing DTT concentration results in increasing PcpC activity, reaching a maximum at approximately 30 mM DTT and remaining high up to 100 mM DTT | Sphingobium sp. UG30 |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.21.4.5 | expression in Escherichia coli | Sphingobium sp. UG30 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.4.5 | additional information | PcpC is not inhibited by the 2,3,5,6-tetrachlorohydroquinone substrate at concentrations 0.3 mM | Sphingobium sp. UG30 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.4.5 | 0.0203 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 50°C | Sphingobium sp. UG30 | |
| 1.21.4.5 | 0.1492 | - |
glutathione | pH 8.7, 50°C | Sphingobium sp. UG30 | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 28245 | - |
calculated from sequence | Sphingobium sp. UG30 |
| 1.21.4.5 | 30000 | - |
SDS-PAGE | Sphingobium sp. UG30 |
| 1.21.4.5 | 56000 | - |
gel filtration | Sphingobium sp. UG30 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.4.5 | 2,3,5,6-tetrachlorohydroquinone + 2 glutathione | Sphingobium sp. UG30 | - |
2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
| 1.21.4.5 | 2,3,6-trichlorohydroquinone + 2 glutathione | Sphingobium sp. UG30 | - |
2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.4.5 | Sphingobium sp. UG30 | Q937X0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.4.5 | - |
Sphingobium sp. UG30 |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 103 | - |
pH 8.7, 50°C | Sphingobium sp. UG30 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.4.5 | 2,3,5,6-tetrachlorohydroquinone + 2 glutathione | - |
Sphingobium sp. UG30 | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
| 1.21.4.5 | 2,3,6-trichlorohydroquinone + 2 glutathione | - |
Sphingobium sp. UG30 | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.21.4.5 | dimer | 2 * 30000, SDS-PAGE | Sphingobium sp. UG30 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.21.4.5 | pcpC | - |
Sphingobium sp. UG30 |
| 1.21.4.5 | tetrachlorohydroquinone reductive dehalogenase | - |
Sphingobium sp. UG30 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 50 | - |
- |
Sphingobium sp. UG30 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 30 | 50 | 30°C: about 30% of maximal activity, 50°C: about 60% of maximal activity | Sphingobium sp. UG30 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 100 | - |
10 min, complete inactivation | Sphingobium sp. UG30 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.4.5 | 48.5 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 50°C | Sphingobium sp. UG30 | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 8.7 | - |
- |
Sphingobium sp. UG30 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.4.5 | 8 | 9.5 | pH 8.0: about 50% of maximal activity, pH 9.5: about 40% of maximal activity | Sphingobium sp. UG30 |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.21.4.5 | Sphingobium sp. UG30 | calculated from sequence | - |
4.99 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.4.5 | 2389 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 50°C | Sphingobium sp. UG30 | |
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