EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 4-hydroxybenzoic acid | 109.7% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | p-coumaric acid | 118% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | syringic acid | 111.4% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | vanillic acid | 125.1% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Vanillin | 118% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 4-hydroxybenzoic acid | 97.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | acetone | 68.3% and 62% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | Al3+ | 83.5% and 43.7% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | Ba2+ | 76.7% residual activity at 2.5 mM with cellobiose as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Cu2+ | complete inhibition at 2.5 mM | Thermothelomyces thermophilus | |
3.2.1.21 | D-glucose | competitive inhibition | Thermothelomyces thermophilus | |
3.2.1.21 | dithiothreitol | 82.5% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | DMSO | 92.9% and 57% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | ethanol | 70.6% and 55.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | Fe2+ | complete inhibition at 2.5 mM | Thermothelomyces thermophilus | |
3.2.1.21 | ferulic acid | 97.4% and 99.1% residual activity at 0.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | gallic acid | 91.1% and 75.3% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | Isopropanol | 66.5-70.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | K+ | 76.2% and 81.9% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | Mg2+ | 62.7% residual activity at 2.5 mM with cellobiose as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Mn2+ | 49.6% and 67.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | Na+ | 70.6% and 57.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | p-coumaric acid | 70.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | PEG 8000 | 67.6% and 58.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | polyvinyl alcohol | 71.2% and 56.8% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | SDS | 82.4% and 60.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | syringaldehyde | 92.8% and 96.4% residual activity at 0.25 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | syringic acid | 95.2% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Tannic acid | complete inhibition at 0.0625 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Triton X-100 | 57.5% and 45% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
3.2.1.21 | vanillic acid | 87.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Vanillin | 94% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
3.2.1.21 | Zn2+ | 63% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.52 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 5.0 and 60°C | Thermothelomyces thermophilus | |
3.2.1.21 | 8.5 | - |
cellobiose | at pH 5.0 and 50°C | Thermothelomyces thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | Li+ | 102.6-128.4% activity at 2.5 mM | Thermothelomyces thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Thermothelomyces thermophilus | - |
- |
- |
3.2.1.21 | Thermothelomyces thermophilus M.7.7 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | Q-Sepharose resin column chromatography and Sephacrly S-100 gel filtration | Thermothelomyces thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermothelomyces thermophilus | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermothelomyces thermophilus M.7.7 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Thermothelomyces thermophilus | 2 beta-D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Thermothelomyces thermophilus M.7.7 | 2 beta-D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | ? | x * 50000, SDS-PAGE | Thermothelomyces thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | BGL50 | - |
Thermothelomyces thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 50 | - |
with cellobiose as substrate | Thermothelomyces thermophilus |
3.2.1.21 | 60 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 60 | - |
the enzyme is very stable at 60°C displaying a half-life of 855.6 min but it is easily denatured above this temperature | Thermothelomyces thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 4.5 | - |
with cellobiose as substrate | Thermothelomyces thermophilus |
3.2.1.21 | 5 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 1.5 | - |
D-glucose | at pH 5.0 and 60°C | Thermothelomyces thermophilus |