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Literature summary extracted from

  • Hagstrom, D.; Hirokawa, H.; Zhang, L.; Radic, Z.; Taylor, P.; Collins, E.S.
    Planarian cholinesterase in vitro characterization of an evolutionarily ancient enzyme to study organophosphorus pesticide toxicity and reactivation (2017), Arch. Toxicol., 91, 2837-2847 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 BW284c51 reversible inhibitor Dugesia japonica
3.1.1.7 Carbaryl low inhibition, irreversible inhibitor Dugesia japonica
3.1.1.7 chlorpyrifos oxon irreversible inhibitor Dugesia japonica
3.1.1.7 diazinon oxon high inhibition, irreversible inhibitor Dugesia japonica
3.1.1.7 dichlorvos irreversible inhibitor Dugesia japonica
3.1.1.7 donepezil reversible inhibitor Dugesia japonica
3.1.1.7 edrophonium reversible inhibitor Dugesia japonica
3.1.1.7 ethopropazine reversible inhibitor Dugesia japonica
3.1.1.7 malaoxon low inhibition, irreversible inhibitor Dugesia japonica
3.1.1.7 additional information the quaternary pyridinium aldoximes, pralidoxime (2-PAM) and a lead zwitterionic oxime (RS194B), are both able to reactivate DjChE ATCh hydrolyzing activity at concentrations of 4 mM. Also NaF can reactivate DjChE activity, and the rate of reactivation shows a linear dependence on fluoride concentration. Reactivation of DjChE is significantly more rapid with 10 mM NaF than with 4 mM 2-PAM Dugesia japonica
3.1.1.7 neostigmine high inhibition, irreversible inhibitor Dugesia japonica
3.1.1.7 paraoxon irreversible inhibitor Dugesia japonica
3.1.1.7 physostigmine irreversible inhibitor Dugesia japonica
3.1.1.7 propidium reversible inhibitor Dugesia japonica
3.1.1.7 pyridostigmine irreversible inhibitor Dugesia japonica
3.1.1.7 tacrine reversible inhibitor Dugesia japonica
3.1.1.8 BW284c51 reversible inhibitor Dugesia japonica
3.1.1.8 Carbaryl low inhibition, irreversible inhibitor Dugesia japonica
3.1.1.8 chlorpyrifos oxon irreversible inhibitor Dugesia japonica
3.1.1.8 diazinon oxon high inhibition, irreversible inhibitor Dugesia japonica
3.1.1.8 dichlorvos irreversible inhibitor Dugesia japonica
3.1.1.8 donepezil reversible inhibitor Dugesia japonica
3.1.1.8 edrophonium reversible inhibitor Dugesia japonica
3.1.1.8 ethopropazine reversible inhibitor Dugesia japonica
3.1.1.8 malaoxon low inhibition, irreversible inhibitor Dugesia japonica
3.1.1.8 additional information the quaternary pyridinium aldoximes, pralidoxime (2-PAM) and a lead zwitterionic oxime (RS194B), are both able to reactivate DjChE ATCh hydrolyzing activity at concentrations of 4 mM. Also NaF can reactivate DjChE activity, and the rate of reactivation shows a linear dependence on fluoride concentration. Reactivation of DjChE is significantly more rapid with 10 mM NaF than with 4 mM 2-PAM Dugesia japonica
3.1.1.8 neostigmine high inhibition, irreversible inhibitor Dugesia japonica
3.1.1.8 paraoxon irreversible inhibitor Dugesia japonica
3.1.1.8 physostigmine irreversible inhibitor Dugesia japonica
3.1.1.8 propidium reversible inhibitor Dugesia japonica
3.1.1.8 pyridostigmine irreversible inhibitor Dugesia japonica
3.1.1.8 tacrine reversible inhibitor Dugesia japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.7 acetylcholine + H2O Dugesia japonica
-
 choline + acetate
-
 ?
3.1.1.8 butyrylcholine + H2O Dugesia japonica
-
 choline + butyrate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Dugesia japonica
-
-
-
3.1.1.8 Dugesia japonica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
 Dugesia japonica choline + acetate
-
 ?
3.1.1.8 butyrylcholine + H2O
-
 Dugesia japonica choline + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 ATCh
-
 Dugesia japonica
3.1.1.7 cholinesterase
-
 Dugesia japonica
3.1.1.7 DjChE
-
 Dugesia japonica
3.1.1.7 More cf. EC 3.1.1.8 Dugesia japonica
3.1.1.8 BTCh
-
 Dugesia japonica
3.1.1.8 cholinesterase
-
 Dugesia japonica
3.1.1.8 DjChE
-
 Dugesia japonica
3.1.1.8 Djche1
-
 Dugesia japonica
3.1.1.8 Djche2
-
 Dugesia japonica
3.1.1.8 More cf. EC 3.1.1.7 Dugesia japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 22
-
 assay at room temperature Dugesia japonica
3.1.1.8 22
-
 assay at room temperature Dugesia japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.4
-
 assay at Dugesia japonica
3.1.1.8 7.4
-
 assay at Dugesia japonica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.7 additional information
-
 additional information inhibition kinetics Dugesia japonica
3.1.1.8 additional information
-
 additional information inhibition kinetics Dugesia japonica

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.02
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate acetylcholine Dugesia japonica BW284c51
3.1.1.7 0.03
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate acetylcholine Dugesia japonica propidium
3.1.1.7 0.031
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate acetylcholine Dugesia japonica edrophonium
3.1.1.7 0.032
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate acetylcholine Dugesia japonica BW284c51
3.1.1.7 0.062
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate acetylcholine Dugesia japonica tacrine
3.1.1.7 0.069
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate acetylcholine Dugesia japonica donepezil
3.1.1.7 0.089
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate acetylcholine Dugesia japonica donepezil
3.1.1.7 0.093
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate acetylcholine Dugesia japonica ethopropazine
3.1.1.7 0.13
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate acetylcholine Dugesia japonica ethopropazine
3.1.1.7 0.14
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate acetylcholine Dugesia japonica tacrine
3.1.1.7 0.16
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate acetylcholine Dugesia japonica edrophonium
3.1.1.7 0.3
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate acetylcholine Dugesia japonica propidium
3.1.1.8 0.023
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate butyrylcholine Dugesia japonica BW284c51
3.1.1.8 0.089
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate butyrylcholine Dugesia japonica BW284c51
3.1.1.8 0.091
-
 pH 7.4, 22°C, crude enzyme, with 0.1 mM substrate butyrylcholine Dugesia japonica ethopropazine
3.1.1.8 0.22
-
 pH 7.4, 22°C, crude enzyme, with 1 mM substrate butyrylcholine Dugesia japonica ethopropazine

General Information

EC Number General Information Comment Organism
3.1.1.7 evolution as one of the most ancient true cholinesterases, DjChE provides insight into the evolution of a hybrid enzyme before the separation into distinct AChE and BChE enzymes found in higher vertebrates Dugesia japonica
3.1.1.7 physiological function the majority of Dugesia japonica cholinesterase (DjChE) activity departs from conventional AChE and BChE classifications Dugesia japonica
3.1.1.8 evolution as one of the most ancient true cholinesterases, DjChE provides insight into the evolution of a hybrid enzyme before the separation into distinct AChE and BChE enzymes found in higher vertebrates Dugesia japonica
3.1.1.8 physiological function the majority of Dugesia japonica cholinesterase (DjChE) activity departs from conventional AChE and BChE classifications Dugesia japonica