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Literature summary extracted from

  • Araujo, M.C.; Assis, C.R.D.; Silva, K.C.C.; Souza, K.S.; Azevedo, R.S.; Alves, M.H.M.E.; Silva, L.C.; Silva, V.L.; Adam, M.L.; Carvalho Junior, L.B.; Souza Bezerra, R.; Oliveira, M.B.M.
    Characterization of brain acetylcholinesterase of bentonic fish Hoplosternum littorale perspectives of application in pesticides and metal ions biomonitoring (2018), Aquat. Toxicol., 205, 213-226 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.7 diagnostics the enzyme has potential as biomarker of pesticides and metal ions Hoplosternum littorale

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 Aldicarb
-
 Hoplosternum littorale
3.1.1.7 As3+
-
 Hoplosternum littorale
3.1.1.7 BW284c51
-
 Hoplosternum littorale
3.1.1.7 Carbaryl
-
 Hoplosternum littorale
3.1.1.7 carbofuran
-
 Hoplosternum littorale
3.1.1.7 Cd2+
-
 Hoplosternum littorale
3.1.1.7 chlorpyrifos
-
 Hoplosternum littorale
3.1.1.7 Cu2+
-
 Hoplosternum littorale
3.1.1.7 diazinon
-
 Hoplosternum littorale
3.1.1.7 dichlorvos
-
 Hoplosternum littorale
3.1.1.7 Diflubenzuron
-
 Hoplosternum littorale
3.1.1.7 eserine
-
 Hoplosternum littorale
3.1.1.7 Hg2+
-
 Hoplosternum littorale
3.1.1.7 iso-ompa
-
 Hoplosternum littorale
3.1.1.7 neostigmine
-
 Hoplosternum littorale
3.1.1.7 novaluron
-
 Hoplosternum littorale
3.1.1.7 parathion-methyl
-
 Hoplosternum littorale
3.1.1.7 Pb2+
-
 Hoplosternum littorale
3.1.1.7 pyriproxyfen
-
 Hoplosternum littorale
3.1.1.7 temephos
-
 Hoplosternum littorale
3.1.1.7 Zn2+
-
 Hoplosternum littorale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 additional information
-
 additional information Michaelis-Menten kinetics Hoplosternum littorale
3.1.1.7 1.47
-
 acetylthiocholine pH 8.0, 30°C Hoplosternum littorale

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.7 additional information no or poor effects by 1 mM of Fe2+, Mn2+, Mg2+, and K+ Hoplosternum littorale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.7 acetylcholine + H2O Hoplosternum littorale
-
 choline + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Hoplosternum littorale
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 brain
-
 Hoplosternum littorale
-
3.1.1.7 central nervous system
-
 Hoplosternum littorale
-
3.1.1.7 neuron
-
 Hoplosternum littorale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
 Hoplosternum littorale choline + acetate
-
 ?
3.1.1.7 acetylthiocholine + H2O
-
 Hoplosternum littorale thiocholine + acetate
-
 ?
3.1.1.7 additional information activity detection with chromogenic reagent 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) Hoplosternum littorale ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Hoplosternum littorale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 30
-
-
 Hoplosternum littorale

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.7 15 45 over 70% of maximal activity within this range Hoplosternum littorale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.7 30
-
 100% activity remaining Hoplosternum littorale
3.1.1.7 40
-
 80% activity remaining Hoplosternum littorale
3.1.1.7 50
-
 no activity remaining Hoplosternum littorale

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.7 8100
-
 acetylthiocholine pH 8.0, 30°C Hoplosternum littorale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 8
-
-
 Hoplosternum littorale

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.7 0.0000145
-
 neostigmine pH 8.0, 30°C Hoplosternum littorale
3.1.1.7 0.0000184
-
 BW284c51 pH 8.0, 30°C Hoplosternum littorale
3.1.1.7 0.0000247
-
 eserine pH 8.0, 30°C Hoplosternum littorale
3.1.1.7 0.000035
-
 iso-ompa pH 8.0, 30°C Hoplosternum littorale

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.0002
-
 pH 8.0, 30°C Hoplosternum littorale eserine
3.1.1.7 0.00034
-
 pH 8.0, 30°C Hoplosternum littorale neostigmine
3.1.1.7 0.00196
-
 pH 8.0, 30°C Hoplosternum littorale BW284c51
3.1.1.7 0.00483
-
 pH 8.0, 30°C Hoplosternum littorale iso-ompa

General Information

EC Number General Information Comment Organism
3.1.1.7 physiological function acetylcholinesterase (AChE) is a serine hydrolase, whose main function is to modulate neurotransmission at cholinergic synapses. It is, therefore, the primary target of some pesticides and heavy metals. Its inhibition in aquatic organisms has been used as an indicator of the presence of these pollutants in water bodies. Acetylcholinesterase is a key enzyme in the regulation of synaptic transmission. AChE promotes the hydrolysis of the neurotransmitter, acetylcholine, to ensure intermittent nerve impulses (which characterize neuronal communication) while preventing acute cholinergic syndrome that can result if this neurotransmitter accumulates in the nerve endings of the central and peripheral nervous system Hoplosternum littorale

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.7 5510
-
 acetylthiocholine pH 8.0, 30°C Hoplosternum littorale