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Literature summary extracted from

  • Shimada, Y.; Watanabe, Y.; Wakinaka, T.; Funeno, Y.; Kubota, M.; Chaiwangsri, T.; Kurihara, S.; Yamamoto, K.; Katayama, T.; Ashida, H.
    alpha-N-acetylglucosaminidase from Bifidobacterium bifidum specifically hydrolyzes alpha-linked N-acetylglucosamine at nonreducing terminus of O-glycan on gastric mucin (2015), Appl. Microbiol. Biotechnol., 99, 3941-3948 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.50 expressed in Escherichia coli BL21(lambdaDE3) cells Bifidobacterium bifidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.50 Cu2+ 5 mM Cu2+ reduces the activity to 10% Bifidobacterium bifidum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.50 extracellular
-
 Bifidobacterium bifidum
-
-
3.2.1.50 membrane a membrane-anchored extracellular enzyme Bifidobacterium bifidum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.50 additional information Ca2+, Mg2+, Zn2+, Mn2+, Ni2+, and Co2+, do not affect enzyme activity at 5 mM Bifidobacterium bifidum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Bifidobacterium bifidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50 HisTrap column chromatography, and Superdex 200 gel filtration Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 4-methylumbelliferyl 4-O-N-acetyl-alpha-D-glucosaminyl-beta-D-galactoside + H2O
-
 Bifidobacterium bifidum N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-galactosaminide
-
 ?
3.2.1.50 4-methylumbelliferyl 6-O-N-acetyl-alpha-D-glucosaminyl-beta-D-galactoside + H2O slow reaction Bifidobacterium bifidum N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-galactosaminide
-
 ?
3.2.1.50 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide + H2O efficient substrate Bifidobacterium bifidum 4-methylumbelliferone + N-acetyl-alpha-D-glucosamine
-
 ?
3.2.1.50 4-nitrophenyl N-acetyl-alpha-D-glucosaminide + H2O slow reaction Bifidobacterium bifidum 4-nitrophenol + N-acetyl-alpha-D-glucosamine
-
 ?
3.2.1.50 additional information no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide, 4-nitrophenyl N-acetyl-alpha-D-galactosaminide, 4-nitrophenyl N-acetyl-beta-D-galactosaminide, 4-nitrophenyl alpha-D-glucosaminide, 4-nitrophenyl beta-D-glucosaminide, 4-nitrophenyl alpha-D-galactosaminide, 4-nitrophenyl beta-D-galactosaminide, 4-nitrophenyl alpha-D-fucosaminide, 4-nitrophenyl beta-D-fucosaminide, 4-nitrophenyl alpha-D-mannosaminide, 4-nitrophenyl beta-D-mannosaminide, 4-nitrophenyl alpha-D-xylosaminide, and 4-nitrophenyl beta-D-xylosaminide. Alpha1,2- and alpha1,3-linked N-acetyl-alpha-D-glucosaminides are resistant to this enzyme Bifidobacterium bifidum ?
-
 ?
3.2.1.50 porcine gastric mucin + H2O the enzyme specifically hydrolyzes alpha-linked N-acetylglucosamine at nonreducing terminus of O-glycan on gastric mucin Bifidobacterium bifidum N-acetyl-alpha-D-glucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.50 ? x * 200000, SDS-PAGE Bifidobacterium bifidum
3.2.1.50 ? x * 212000, calculated from amino acid sequence Bifidobacterium bifidum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.50 AgnB
-
 Bifidobacterium bifidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.50 50
-
-
 Bifidobacterium bifidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.50 40
-
 the enzyme is stable up to 40°C Bifidobacterium bifidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 5.5
-
-
 Bifidobacterium bifidum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.50 4 6.5 more than 80 % activity is detected between pH 4.0 and 6.5 Bifidobacterium bifidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.50 5 9.5 the enzyme is stable between pH 5.0 and 9.5 Bifidobacterium bifidum

General Information

EC Number General Information Comment Organism
3.2.1.50 physiological function the enzyme is important for assimilation of gastroduodenal mucin by Bacillus bifidum and also applicable to production of prebiotic oligosaccharides from porcine gastric mucin Bifidobacterium bifidum