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Literature summary extracted from

  • Huang, Y.; Zheng, X.; Pilgaard, B.; Holck, J.; Muschiol, J.; Li, S.; Lange, L.
    Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea (2019), Appl. Microbiol. Biotechnol., 103, 777-791 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 heterologously expressed in Pichia pastoris Rhizophlyctis rosea
3.2.1.37 heterologously expressed in Pichia pastoris Rhizophlyctis rosea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 D-xylose this enzyme shows low product inhibition and retained 51% activity in the presence of 100 mM xylose Rhizophlyctis rosea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan Km: 18.83 mg/ml, pH 7, 50°C, large enzyme form RrXyn11A L Rhizophlyctis rosea
3.2.1.8 additional information
-
 beechwood xylan Km: 21.63 mg/ml, pH 7, 40°C, small enzyme form RrXyn11A S Rhizophlyctis rosea
3.2.1.37 1.34
-
 4-nitrophenyl beta-D-xylopyranoside 25°C, pH 7.0 Rhizophlyctis rosea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 31000
-
 calculated from sequence Rhizophlyctis rosea
3.2.1.37 39000
-
 calculated from sequence Rhizophlyctis rosea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Rhizophlyctis rosea A0A2U8LMQ3
-
-
3.2.1.8 Rhizophlyctis rosea NBRC 105426 A0A2U8LMQ3
-
-
3.2.1.37 Rhizophlyctis rosea A0A2U8LMQ1
-
-
3.2.1.37 Rhizophlyctis rosea NBRC 105426 A0A2U8LMQ1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 additional information the enzyme is not glycosylated Rhizophlyctis rosea
3.2.1.37 additional information there is no predicted N-glycosylation site for RrXyl43A Rhizophlyctis rosea

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Rhizophlyctis rosea
3.2.1.37
-
 Rhizophlyctis rosea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl beta-D-xylopyranoside
-
 Rhizophlyctis rosea 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.8 4-nitrophenyl beta-D-xylopyranoside
-
 Rhizophlyctis rosea NBRC 105426 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.8 azurin-cross-linked arabinoxylan + H2O
-
 Rhizophlyctis rosea ?
-
 ?
3.2.1.8 azurin-cross-linked arabinoxylan + H2O
-
 Rhizophlyctis rosea NBRC 105426 ?
-
 ?
3.2.1.8 azurin-cross-linked xylan + H2O
-
 Rhizophlyctis rosea ?
-
 ?
3.2.1.8 azurin-cross-linked xylan + H2O
-
 Rhizophlyctis rosea NBRC 105426 ?
-
 ?
3.2.1.8 beechwood xylan + H2O
-
 Rhizophlyctis rosea ?
-
 ?
3.2.1.8 beechwood xylan + H2O
-
 Rhizophlyctis rosea NBRC 105426 ?
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O low activity Rhizophlyctis rosea 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O low activity Rhizophlyctis rosea NBRC 105426 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Rhizophlyctis rosea 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Rhizophlyctis rosea NBRC 105426 4-nitrophenol + beta-D-xylopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 GH11 xylanase
-
 Rhizophlyctis rosea
3.2.1.8 RrXyn11A
-
 Rhizophlyctis rosea
3.2.1.37 GH43 xylosidase
-
 Rhizophlyctis rosea
3.2.1.37 RrXyl43A
-
 Rhizophlyctis rosea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 40
-
 small enzyme form RrXyn11A S Rhizophlyctis rosea
3.2.1.8 50
-
 large enzyme form RrXyn11A L Rhizophlyctis rosea
3.2.1.37 25
-
-
 Rhizophlyctis rosea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 1 h, 56% residual activity (small enzyme form RrXyn11A S), 1 h, 65% residual activity (larger enzyme form RrXyn11A L) Rhizophlyctis rosea
3.2.1.37 40
-
 1 h, 72% residual activity Rhizophlyctis rosea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 54.8
-
 beechwood xylan pH 7, 50°C, large enzyme form RrXyn11A L Rhizophlyctis rosea
3.2.1.8 106.25
-
 beechwood xylan pH 7, 40°C, small enzyme form RrXyn11A S Rhizophlyctis rosea
3.2.1.37 21.2
-
 4-nitrophenyl beta-D-xylopyranoside 25°C, pH 7.0 Rhizophlyctis rosea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
-
 Rhizophlyctis rosea
3.2.1.37 7
-
-
 Rhizophlyctis rosea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4 8 stable Rhizophlyctis rosea
3.2.1.37 5 8 stable Rhizophlyctis rosea